20 g/L is the same as 20 mg/ml, isn't it? That does not seem particularly high to me.
Why not try 200 g/L? As a variant to this, you could just try doing 10:1 prot:ppt drops, and allowing the protein concentration to increase in situ. In my limited experience, however, this is not the same as setting up drops 1:1 with more concentrated protein, for reasons unknown to me. I wonder whether that is the experience of others on the BB as well? JPK
