I second Jim's suggestion of concentrating the protein. You should see a third to half of your crystallization drops produce precipitant. If you are below that your concentration is too low. Most of our structural genomics targets crystallize at concentrations above 20 mg/mL and several as high as 75-100 mg/mL.
Thomas Edwards Emerald BioStructures Seattle Structural Genomics Center for Infectious Disease (SSGCID) From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jim Pflugrath Sent: Friday, March 05, 2010 8:10 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] crystallization of a macromolecular complex 20 g/L is the same as 20 mg/ml, isn't it? That does not seem particularly high to me. Why not try 200 g/L? ________________________________ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jan Rash Sent: Friday, March 05, 2010 8:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] crystallization of a macromolecular complex Dear All, This is about the crystallization of the macromolecular complex which is highly soluble and shows no signs of the aggregation (even at high concentration). We have tried several salts, precipitants and even high protein concentration (around 20g/L) for its crystallization without any genuine hit. Any suggestions for growing the crystals of this macromolecular complex will be highly appreciated. Thanks, Jan
