Hi,
In our lab we have background on both instruments, 2 Biorad and 2
Aktas. We got problems on both, minor ones on Aktas, major ones on
Biorads, with expensive fixing on Aktas and cheaper fixings on
Biorads, but efficient fixing on Aktas and unsolved problems on
Biorads. Actually, first Biorad w
Dear CCP4ers
I am trying to make a (comprehensive?) list of examples of small and
medium-size (< ~300kDa) proteins whose crystal structure was solved by
using EM reconstructions as a starting model.
Can anybody direct me to relevant articles in the literature?
Thank you
--
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Jhon Thomas wrote:
Hi all
Dear Jhon,
Thanks for your email and apologies for the problem. Fedora 6 was not
available to us when we built the packages, but hopefully we can sort
you problem out. It certainly will be supported fully in future releases.
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Hi, all
I am working with this protein that only gives very thin needle crystals
(25micron by 25 micron by 100-200micron) despite of various atempts to make
it bigger, I am wondering if anybody here has any information as to where I
can find a micro-focus beamline that will work on tiny needle cr
On Fri, 2007-07-20 at 11:02 -0400, Peng Xie wrote:
> Hi, all
>
> I am working with this protein that only gives very thin needle
> crystals (25micron by 25 micron by 100-200micron) despite of various
> atempts to make it bigger, I am wondering if anybody here has any
> information as to where I c
GM/CA-CAT beamlines at APS. Go to
http://www.gmca.anl.gov/userprogram/applying.html
to apply for beamtime.
We provide 5um, 10um, 20um stable beam with fluxes of 1-2x10**11.
Mini-beam (our terminology) has proven itself indispensable for number
of projects covering wide range of crystal diffraction
Hi Peng,
I am not sure where to go in the US, but the microfocus beamline ID23-2
at the ESRF is the place that very well fit your case (Please see the two
publications below)!
Regards,
Nazzareno.
\
1: Dimasi N, Flot D, Dupeux F, Marquez JA. Expression, crystallization
and X-ray data collectio
Hi,
On Jul 20, 2007, at 5:02 PM, Peng Xie wrote:
it has been a big concern that the tiny seed crystals I collected
on the hair for streak seeding will be wiped out when I drag the
hair through a layer of oil used for microbatch to seed new drops.
It will be great if anybody has some hands
Two hopefully useful remarks:
a. Most if not all beamlines at ESRF/SLS (and presumably APS/ALS/...)
would handle 25x25x200 micron needles without any sweating.
b. Don't forget ID13 of ESRF which as far as I know still has the
prototype micro-diffractometer built at EMBL back in the 20th cen
very true ...and that even goes for the BMs at ESRF!
AT bm14 we have the next generation microdiffractometer (the MD2) that was
pioneered at ID13 at the ESRF (which is also in action at SLS, APS and i believe
ALS either soon or already) and would safely say crystals of the order of
25x25x200 as ta
Thank you so much for all you guys' replies, It looks like APS is a
realistic option, I have been to 23ID D before, I didn't know they now have
the micro focus capability. Thanks again for all your help!
Best,
Peng
On 7/20/07, Martin Austin Walsh <[EMAIL PROTECTED]> wrote:
very true ...and t
Dear all,
I am refining a structure at 2.0 A. The water molecules have been added
using arp/warp resulting Rwork/Rfree=21/26% (about 370 HOH for 360
residues). After 10 cycles of refmac refinement (wt 0.3), Rwork/Rfree went
up about 1.5% to 22.5/27.5%. I did some minor adjustments and add/del
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