Hi Peng, I am not sure where to go in the US, but the microfocus beamline ID23-2 at the ESRF is the place that very well fit your case (Please see the two publications below)!
Regards, Nazzareno. \ 1: Dimasi N, Flot D, Dupeux F, Marquez JA. Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):204-8. 2: Marquez JA, Galfre E, Dupeux F, Flot D, Moran O, Dimasi N. The crystal structure of the extracellular domain of the inhibitor receptor expressed on myeloid cells IREM-1. J Mol Biol. 2007 Mar 23;367(2):310-8. On 7/20/07, Peng Xie <[EMAIL PROTECTED]> wrote:
Hi, all I am working with this protein that only gives very thin needle crystals (25micron by 25 micron by 100-200micron) despite of various atempts to make it bigger, I am wondering if anybody here has any information as to where I can find a micro-focus beamline that will work on tiny needle crystals of this size in US. Also, I tried without any luck to do microseeding with my crystals using microbatch method which is the only method that will give me crystals, it has been a big concern that the tiny seed crystals I collected on the hair for streak seeding will be wiped out when I drag the hair through a layer of oil used for microbatch to seed new drops. It will be great if anybody has some hands on experience on doing microseeding in microbatch can give me some insights on this problem. Thanks you very much in advance for all your help! Best, Peng
