Two hopefully useful remarks:
a. Most if not all beamlines at ESRF/SLS (and presumably APS/ALS/...)
would handle 25x25x200 micron needles without any sweating.
b. Don't forget ID13 of ESRF which as far as I know still has the
prototype micro-diffractometer built at EMBL back in the 20th century.
There is test beamtime still available and you might want to give
that a go, as well as to the absolutely superb ID23-2.
I am getting sentimental now ... but I will refrain from listing
publications from the dark ages of the '90s ;-)))
Tassos
On 20 Jul 2007, at 17:51, Nazzareno Dimasi wrote:
Hi Peng,
I am not sure where to go in the US, but the microfocus beamline
ID23-2
at the ESRF is the place that very well fit your case (Please see
the two
publications below)!
Regards,
Nazzareno.
\
1: Dimasi N, Flot D, Dupeux F, Marquez JA. Expression, crystallization
and X-ray data collection from microcrystals of the extracellular
domain
of the human inhibitory receptor expressed on myeloid cells
IREM-1. Acta Crystallograph Sect F Struct Biol Cryst Commun.
2007 Mar 1;63(Pt 3):204-8.
2: Marquez JA, Galfre E, Dupeux F, Flot D, Moran O, Dimasi N. The
crystal
structure of the extracellular domain of the inhibitor receptor
expressed on myeloid cells IREM-1. J Mol Biol. 2007 Mar 23;367(2):
310-8.
On 7/20/07, Peng Xie <[EMAIL PROTECTED]> wrote:
Hi, all
I am working with this protein that only gives very thin needle
crystals (25micron by 25 micron by 100-200micron) despite of
various atempts to make it bigger, I am wondering if anybody here
has any information as to where I can find a micro-focus beamline
that will work on tiny needle crystals of this size in US.
Also, I tried without any luck to do microseeding with my crystals
using microbatch method which is the only method that will give me
crystals, it has been a big concern that the tiny seed crystals I
collected on the hair for streak seeding will be wiped out when I
drag the hair through a layer of oil used for microbatch to seed
new drops. It will be great if anybody has some hands on experience
on doing microseeding in microbatch can give me some insights on
this problem.
Thanks you very much in advance for all your help!
Best,
Peng
