. However, phaser gave P22121, RFZ=3.7, TFZ=4.8, park=4, LLG=26, LLG=24.
Thanks
peter
Date: Tue, 24 Nov 2009 20:37:01 -0500
From: mjvdwo...@netscape.net
Subject: Re: [ccp4bb] Methylation of macromolecular complexes
To: CCP4BB@JISCMAIL.AC.UK
There is a reasonable experiment you can do:
Take the
[mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jan
Rash
Sent: Tuesday, November 24, 2009 11:46 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Methylation of macromolecular complexes
Hi all,
What I really want to know is whether macromolecular complexes ( more than
200kDa) can tolerate methylation
nough
that direct methods work very well.
Mark
-Original Message-
From: Sean Seaver
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, Nov 24, 2009 5:13 pm
Subject: Re: [ccp4bb] Methylation of macromolecular complexes
>I have a small complex, one component is 13 kDa with structure available
>I have a small complex, one component is 13 kDa with structure available
and the other is 7 kDa, which could not be able to grew crystals after lots
of efforts. It grew crystals after methylation and diffracted to 2.3 A,
however, I could not be able to solve the structure using the structure of
th
Date: Tue, 24 Nov 2009 18:27:49 +0100
From: jan...@googlemail.com
Subject: [ccp4bb] Methylation of macromolecular complexes
To: CCP4BB@JISCMAIL.AC.UK
Hi all,
I have a question regarding the methylation of macromolecular complexes
any idea and suggestions how to
solve the structure using the data and the template avaliable.
Many thanks for your help.
Peter
Date: Tue, 24 Nov 2009 18:27:49 +0100
From: jan...@googlemail.com
Subject: [ccp4bb] Methylation of macromolecular complexes
To: CCP4BB@JISCMAIL.AC.UK
Hi all
cken
diese E-Mail nur aus, wenn unbedingt notwendig! Please consider the
environment before printing this email!
_
Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von Jan
Rash
Gesendet: Dienstag, 24. November 2009 18:28
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Me
Mail nur aus, wenn unbedingt notwendig! Please consider
> the environment before printing this email!
>
>
> --
>
> *Von:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *Im Auftrag von
> *Jan Rash
> *Gesendet:* Dienstag, 24. November 2009 18:28
Jan Rash wrote:
> Hi all,
> I have a question regarding the methylation of macromolecular
> complexes. Is there any report where a macromolecular complex has been
> successfully methylated and later on crystallized. Is methylation a
> method of choice to chemically modify macromolecular complexes
Hi all,
I have a question regarding the methylation of macromolecular complexes. Is
there any report where a macromolecular complex has been successfully
methylated and later on crystallized. Is methylation a method of choice to
chemically modify macromolecular complexes where the entropical
contr
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