Jan Rash wrote: > Hi all, > I have a question regarding the methylation of macromolecular > complexes. Is there any report where a macromolecular complex has been > successfully methylated and later on crystallized. Is methylation a > method of choice to chemically modify macromolecular complexes where > the entropical contributions by lysines might be a cause for not > obtaining crystals, or is there any other better ways? > > thanks, > Jan Rash You could also try to use the surface entropy reduction server (http://nihserver.mbi.ucla.edu/SER/) for identification of sites that are most suitable for mutation designed to enhance crystallizability. Methylation is probably more invasive.
-- Tim Schulte Ruhr-Universität Bochum LS Biophysik AG Proteinkristallographie Universitätsstr. 150 44780 Bochum Germany Tel. +49-234-32-25754
