Hi all, What I really want to know is whether macromolecular complexes ( more than 200kDa) can tolerate methylation? thanks, Jan Rash
2009/11/24 Hüsnü Topal <huesnue.to...@rub.de> > Hi Jan, > > > > Maybe this link > http://www.jenabioscience.com/cms/en/1/catalog/529_jbs_methylation_kit.htmlor > these references helps… > > > > References > [1] Kim *et al. *(2008) Large-scale evaluation of protein reductive > methylation for improving protein crystallization. *Nature Methods* *5*, > 853. > > [2] Fogg *et al*. (2006) Application of the use of high-throughput > technologies to the determination of protein structures of bacterial and > viral pathogens. *Acta Cryst*. D*62*:1196. > > [3] Walter *et al*. (2006) Lysine methylation as a routine rescue strategy > for protein crystallization. *Structure* *14*:1617. > > I’m not sure if this answers your question. Good luck. > > > > best regards, > > Hüsnü Topal > > Ruhr-Universität Bochum > Institut für Physiologische Chemie, MA 2/141 > Abteilung für Biochemie supramolekularer Systeme > Universitätsstr. 150 > > 44801 Bochum, Germany > > phone: +49 (0) 234 32 24934 > > Internet: http://www.ruhr-uni-bochum.de/physiolchem/steegborn/ > > Diese E-Mail enthält vertrauliche und/oder rechtlich geschützte > Informationen. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail > irrtümlich erhalten haben, informieren Sie bitte sofort den Absender und > vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte > Weitergabe dieser Mail ist nicht gestattet. > > This e-mail may contain confidential and/or privileged information. If you > are not the intended recipient (or have received this e-mail in error) > please notify the sender immediately and destroy this e-mail. Any > unauthorized copying, disclosure or distribution of the material in this > e-mail is strictly forbidden. > > J *Go green* Bitte denken Sie an die Umwelt und den Klimaschutz und > drucken diese E-Mail nur aus, wenn unbedingt notwendig! Please consider > the environment before printing this email! > > > ------------------------------ > > *Von:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *Im Auftrag von > *Jan Rash > *Gesendet:* Dienstag, 24. November 2009 18:28 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [ccp4bb] Methylation of macromolecular complexes > > > > Hi all, > I have a question regarding the methylation of macromolecular complexes. > Is there any report where a macromolecular complex has been successfully > methylated and later on crystallized. Is methylation a method of choice to > chemically modify macromolecular complexes where the entropical > contributions by lysines might be a cause for not obtaining crystals, or is > there any other better ways? > > thanks, > Jan Rash >
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