Hi all,
I have a small complex, one component is 13 kDa with structure available and
the other is 7 kDa, which could not be able to grew crystals after lots of
efforts. It grew crystals after methylation and diffracted to 2.3 A, however, I
could not be able to solve the structure using the structure of the big
component as a template for molecular replacement, and heavy atoms soaking was
not successful. I plan to do selenomethionine expression next. Does the
methylation change protein structures a lot? otherwise, why does molecular
replacement not work? I would much appreciate any idea and suggestions how to
solve the structure using the data and the template avaliable.
Many thanks for your help.
Peter
Date: Tue, 24 Nov 2009 18:27:49 +0100
From: jan...@googlemail.com
Subject: [ccp4bb] Methylation of macromolecular complexes
To: CCP4BB@JISCMAIL.AC.UK
Hi all,
I have a question regarding the methylation of macromolecular complexes. Is
there any report where a macromolecular complex has been successfully
methylated and later on crystallized. Is methylation a method of choice to
chemically modify macromolecular complexes where the entropical contributions
by lysines might be a cause for not obtaining crystals, or is there any other
better ways?
thanks,
Jan Rash
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