Hi Jan,
Maybe this link <http://www.jenabioscience.com/cms/en/1/catalog/529_jbs_methylation_kit.html > http://www.jenabioscience.com/cms/en/1/catalog/529_jbs_methylation_kit.html or these references helps References [1] Kim et al. (2008) Large-scale evaluation of protein reductive methylation for improving protein crystallization. Nature Methods 5, 853. [2] Fogg et al. (2006) Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. Acta Cryst. D62:1196. [3] Walter et al. (2006) Lysine methylation as a routine rescue strategy for protein crystallization. Structure 14:1617. Im not sure if this answers your question. Good luck. best regards, Hüsnü Topal Ruhr-Universität Bochum Institut für Physiologische Chemie, MA 2/141 Abteilung für Biochemie supramolekularer Systeme Universitätsstr. 150 44801 Bochum, Germany phone: +49 (0) 234 32 24934 Internet: <http://www.ruhr-uni-bochum.de/physiolchem/steegborn/> http://www.ruhr-uni-bochum.de/physiolchem/steegborn/ Diese E-Mail enthält vertrauliche und/oder rechtlich geschützte Informationen. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtümlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. This e-mail may contain confidential and/or privileged information. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden. J Go green Bitte denken Sie an die Umwelt und den Klimaschutz und drucken diese E-Mail nur aus, wenn unbedingt notwendig! Please consider the environment before printing this email! _____ Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von Jan Rash Gesendet: Dienstag, 24. November 2009 18:28 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Methylation of macromolecular complexes Hi all, I have a question regarding the methylation of macromolecular complexes. Is there any report where a macromolecular complex has been successfully methylated and later on crystallized. Is methylation a method of choice to chemically modify macromolecular complexes where the entropical contributions by lysines might be a cause for not obtaining crystals, or is there any other better ways? thanks, Jan Rash
<<attachment: image002.gif>>
