Re: [ccp4bb] Connecting DNA between asymmetric units?

2023-08-01 Thread Ronnie Berntsson
be bigger to accommodate the 60 bases long DNA). I’ll think about it some more.. Best wishes, Ronnie -- Ronnie Berntsson, PhD Chair of the Young Academy of Sweden Department of Medical Biochemistry and Biophysics, Umeå University 90187 Umeå, Sweden e-mail: ronnie.bernts...@umu.se

[ccp4bb] Connecting DNA between asymmetric units?

2023-07-31 Thread Ronnie Berntsson
know if any clarification is needed. Best wishes, Ronnie -- Ronnie Berntsson, PhD Chair of the Young Academy of Sweden Department of Medical Biochemistry and Biophysics, Umeå University 90187 Umeå, Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone:

Re: [ccp4bb] open review?

2022-06-22 Thread Ronnie Berntsson
Hi James, I agree that it’sa good suggestion. In Sweden when you apply to any of the publicly funded grant agencies the application is automatically in the public domain. This means that anyone can ask to see your proposal and the agency is obliged to send it out to the people asking for it. I

[ccp4bb] How to make an isopeptide bond?

2022-03-16 Thread Ronnie Berntsson
either, so I was hoping that someone here might know of an (preferably easy) way to do this? Best wishes, Ronnie -- Ronnie Berntsson, PhD Member of the Young Academy of Sweden Chair of the Swedish Society for Biochemistry, Biophysics and Molecular biology Department of Medical

[ccp4bb] Postdoc fellowship available to study assembly and structure of G+ T4SS at Umeå University, Sweden

2021-01-13 Thread Ronnie Berntsson
-- Ronnie Berntsson, PhD Assistant Professor, Wallenberg Molecular Medicine Fellow Member of the Young Academy of Sweden Department of Medical Biochemistry and Biophysics, Umeå University 90187 Umeå, Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> web:

[ccp4bb] Postdoctoral fellowship available for studies of adhesion in bacterial conjugation

2019-07-02 Thread Ronnie Berntsson
Dear all, A postdoctoral fellowship is available in my group at Umeå University, Sweden (https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/). Our lab studies bacterial conjugation via Type 4 Secretion Systems, with a special focus on gram-positive bacteria. The open position

[ccp4bb] PhD studentship at Umeå University working on Type 4 Secretion Systems

2019-03-29 Thread Ronnie Berntsson
ronnie.bernts...@umu.se Best wishes, Ronnie -- Ronnie Berntsson, PhD Associate Senior Lecturer Wallenberg Molecular Medicine Fellow Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone:

[ccp4bb] Postdoctoral fellowships to study Type 4 Secretion System at Umeå University, Sweden

2019-03-27 Thread Ronnie Berntsson
r more information about the research or other details, do not hesitate to contact Ronnie Berntsson. Email: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> Webpage: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/ -- Ronnie Berntsson, PhD Associate Senior Lecture

[ccp4bb] 2 postdoctoral fellowships available to study Type 4 Secretion Systems at Umeå University, Sweden

2018-12-17 Thread Ronnie Berntsson
for an interview. Starting date according to agreement. For more information, contact Dr. Ronnie Berntsson. Email: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> Webpage: https://www.biostruct.umu.se/principal-investigators/ronn

[ccp4bb] PhD position available in Structural Biology of Type 4 Secretion Systems at Umeå University, Sweden

2018-10-30 Thread Ronnie Berntsson
.se>. Applications must be sent in via the online recruitment system, with a deadline at 7th of December. Regards, -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronn

[ccp4bb] Postdoc position in membran protein structural biology

2017-12-21 Thread Ronnie Berntsson
Please also see our websites: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/ http://www.medchem.umu.se/english/research/principal-investigators/berntsson-ronnie/ With best regards, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Bioc

[ccp4bb] PhD student position available at Umeå University

2017-02-20 Thread Ronnie Berntsson
the online system and not directly to me. Best wishes, -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone: +46 90 7865235

[ccp4bb] Choice of stereomicroscope

2015-03-27 Thread Ronnie Berntsson
. Do any of you have other suggestions regarding the possibility of adding UV to a stereomicroscope? Suggestions and thoughts are more than welcome! Thanks, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå

[ccp4bb] Post-doctoral position at Umeå University, Sweden

2015-03-27 Thread Ronnie Berntsson
Dear all, A post-doctoral position is available in the group of Dr. Ronnie Berntsson at the Department of Medical Biochemistry and Biophysics, Umeå University, Sweden. The project will concern structural and functional characterisation of the adhesion proteins involved in type 4 secretions

[ccp4bb] Postdoctoral position in the Stenmark lab at Stockholm University

2014-12-18 Thread Ronnie Berntsson
Dear all, I’m posting this postdoctoral ad on behalf of Associate Professor Pål Stenmark. Please do not respond to me, but to struct...@dbb.su.se Best wishes, Ronnie Postdoctoral position in structural biology at Stockholm University The Stenmark group has two ma

Re: [ccp4bb] Off topic Chromatography systems

2014-02-19 Thread Ronnie Berntsson
Agilent also has some decent systems. Cheers, Ronnie -- Ronnie Berntsson, PhD PostDoctoral Fellow Department of Biochemistry and Biophysics Arrhenius Laboratories for Natural Sciences Stockholm University 10691 Stockholm Sweden On 18 Feb 2014, at 17:15 , Scott Pegan wrote: >

[ccp4bb] Postdoctoral position in structural biology at Stockholm University

2014-02-18 Thread Ronnie Berntsson
I'm posting this on behalf of Prof. Pål Stenmark. Please direct all correspondence regarding this position to the email specified below (struct...@dbb.su.se). Cheers, Ronnie Berntsson The Stenmark group study novel cancer targets involved within human nucleotide metabolism. This proje

Re: [ccp4bb] Coot 0.7 Saving Dialog opens behind main window

2012-10-30 Thread Ronnie Berntsson
Yes, it's still happening on Mountain Lion. Cheers, Ronnie -- Dr. Ronnie Berntsson PostDoctoral Fellow Department of Biochemistry and Biophysics Arrhenius Laboratories for Natural Sciences Stockholm University 10691 Stockholm Sweden On Oct 30, 2012, at 16:51 , Antony Oliver

Re: [ccp4bb] Checking in dry shippers?

2012-04-18 Thread Ronnie Berntsson
well for the past years, no hiccups. Cheers, Ronnie Berntsson On Apr 18, 2012, at 10:06 , herman.schreu...@sanofi.com wrote: > (very last spam) > At a certain moment, we were flying once or twice a month, so by then I > thought they would know dewars... > It is extremely depending o

Re: [ccp4bb] summary: off topic: advice for crystallography workstation/server

2010-12-16 Thread Ronnie Berntsson
how? Would it need advanced setup and >>> maintenance, or would it be possible to jsut set up a number of user >>> accounts in OS X Server, and let it run? I'm reasonably computer >>> savvy, but haven't really done someth

[ccp4bb] off topic: advice for crystallography workstation/server

2010-12-15 Thread Ronnie Berntsson
would very much appreciate your advice or personal experiences regarding this matter. I know that I could probably get a cheaper computer if I went for a pc with linux, but I have more experience with OS X, and would therefore want to stay with it. Thank you in advance, Ronnie

Re: [ccp4bb] High Rmerge with thin frames

2010-11-05 Thread Ronnie Berntsson
and would like to maximize the potential data quality from our weak diffracting crystals. Any input on this would be greatly appreciated! Cheers, Ronnie Berntsson On Nov 5, 2010, at 16:16, Anastassis Perrakis wrote: > three additional points: > > 1. > >> OTOH, if "The di

Re: [ccp4bb] update on SeMet production

2010-03-23 Thread Ronnie Berntsson
Hi Tim, Lactococcus lactis can also produce SeMet proteins, see: Berntsson et al. Selenomethionine incorporation in proteins expressed in Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7 Cheers, Ronnie On Mar 23, 2010, at 14:40, Tim Gruene wrote: > Dear all, > > I already receiv

Re: [ccp4bb] off topic: multiple structural sequence alignment

2010-01-15 Thread Ronnie Berntsson
nie, > > have you tried the java program 'indonesia' which also contains a > 3D-alignment/sequence comparison tool: > > http://xray.bmc.uu.se/dennis/ > > Best regards, > Bart > > Ronnie Berntsson wrote: >> Dear all, >> A bit off the topic que

[ccp4bb] off topic: multiple structural sequence alignment

2010-01-12 Thread Ronnie Berntsson
very grateful. Cheers, Ronnie Berntsson

Re: [ccp4bb] Minimum hardware requirement of Mac OSX for CCP4

2009-10-27 Thread Ronnie Berntsson
ing machine for crystallography, and even though it is a couple of years old there is basically nothing it still working fine with all software. So a new iMac will work just fine. Cheers, Ronnie Berntsson On Oct 27, 2009, at 13:06, J. Attarataya wrote: Dear Sir, I am a new postgraduate fro

Re: [ccp4bb] How to express a >95KD FAD protein

2009-06-30 Thread Ronnie Berntsson
ophys Acta (2003) 97-108, Geertsma and Poolman. Nat Methods (2007) 705-7, Kunji et al. Curr Opin Biotechnol (2005) 546-51. Cheers, Ronnie Berntsson On Jun 29, 2009, at 23:54, Yong, Wei wrote: Dear all, I know that there are a lot of experts having experience in expressing a big protein in E

Re: [ccp4bb] XDS and ESRF ID23-1 images

2009-03-27 Thread Ronnie Berntsson
This might be a bit too obvious, but have you checked that the path to the images is correct? The path written in the input files generated on the beamline reflects where the images were located in the file tree there. Does it match where you have the files on your own computer? Cheers, Ron

Re: [ccp4bb] Twin refinement not working in CCP4 6.1.1

2009-03-02 Thread Ronnie Berntsson
I have the same problem when running the latest ccp4 (6.1.1 on os x, updated 1 hour ago via fink). Only way I managed to circumvent it is to add the TWIN keyword in the command file as well.. Cheers, Ronnie Berntsson On Mar 2, 2009, at 16:06, Carr, SB (Stephen) wrote: Dear CCP4BB, I have

Re: [ccp4bb] dry shipper on airplaine

2008-05-27 Thread Ronnie Berntsson
In my experience it is still possible to do so, but that rather depends from where you are flying. From my own experience it is always good to contact the airport in question well in advance, partly to check with them that it is ok, partly to let them know when you intend to fly. This tends

Re: [ccp4bb] font size CCP4i

2008-05-23 Thread Ronnie Berntsson
-- Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm - Ronnie Berntsson Ph.D. Student Biochemistry-Enzymology University of Groningen Nijenborgh 4, 9747 AG Groningen, The Netherlands telephone: +31 50 363 4195 telefax: +31 50 363 4165 e-mail: [EM

Re: [ccp4bb] arp/warp and refmac problem on os x

2008-04-24 Thread Ronnie Berntsson
Thanks for a very quick answer, it indeed solved the problem. Cheers, Ronnie On Apr 24, 2008, at 4:55 PM, Chris Richardson wrote: On 24 Apr 2008, at 3:38 PM, Ronnie Berntsson wrote: I've got a rather weird problem when trying to run arp/warp. When trying to run arp/warp (latest ve

[ccp4bb] arp/warp and refmac problem on os x

2008-04-24 Thread Ronnie Berntsson
d ccp4 and refmac via fink. All other CCP4 programs work fine and Refmac runs normally as well, it's only when it's called from arp/warp that something goes wrong.. All ideas to fix this problem are most welcome. Cheers, Ronnie Berntsson

Re: [ccp4bb] crashing-out protein eluted from Nickel column

2008-02-18 Thread Ronnie Berntsson
But if someone can help me more, I will thank!!! Thanks a lot! Juliana Abra sua conta no Yahoo! Mail, o único sem limite de espaço para armazenamento! Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute & Ze

Re: [ccp4bb] microsoft 3-button wheel mouse with OS X 10.5

2008-02-11 Thread Ronnie Berntsson
Hi, These commands does maybe not give you a full featured focus-follows- mouse, but it certainly makes your life easier if you work a lot in OS X and X11.. w defaults write com.apple.x11 wm_ffm -bool false defaults write com.apple.x11 wm_click_through -bool true These two commands should t

Re: [ccp4bb] problem on protein precipitation

2008-01-24 Thread Ronnie Berntsson
pH 7.6. Glycerol is always present during the purification. We do have high salt (500mM) in the buffer. Thank you for you input in advance, Lei Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute & Zernike

[ccp4bb] Postdoc position at Groningen University

2008-01-11 Thread Ronnie Berntsson
to apply, please email a current CV including a brief description of your research interests and accomplishments, and the names and addresses of two referees to Bert Poolman ([EMAIL PROTECTED]) or Dirk Slotboom ([EMAIL PROTECTED]). Please do not respond to me. Thank you. Ronnie Berntsson

Re: [ccp4bb] Apple OS 10.5 and X11

2007-11-06 Thread Ronnie Berntsson
7) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: [EMAIL PROTECTED] Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute & Zernike

Re: [ccp4bb] paper from mol microbiol

2007-10-20 Thread Ronnie Berntsson
I have recieved the paper. Thanks a lot, Ronnie - Ronnie Berntsson Ph.D. Student Biochemistry-Enzymology University of Groningen Nijenborgh 4, 9747 AG Groningen, The Netherlands telephone: +31 50 363 4195 telefax: +31 50 363 4165 e-mail: [EMAIL PROTECTED] homepage: http

[ccp4bb] paper from mol microbiol

2007-10-19 Thread Ronnie Berntsson
1997 and onwards, and unfortunately the librarys printed copy is somewhat difficult to localize... If someone has it lying around I'd be very grateful! With regards, Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology

Re: [ccp4bb] SciVee - YouTube for scientist

2007-08-23 Thread Ronnie Berntsson
Hi all, Great idea! As being reasonably new to the field, I would definitely welcome those videos. Not that I don't read Acta D papers, but it is always nice to hear the talks directly, and would certainly be quite educational. Cheers, Ronnie Berntsson -- Ph.D. St

Re: [ccp4bb] SciVee - YouTube for scientist

2007-08-23 Thread Ronnie Berntsson
Hi all, Great idea! As being relatively new to the field, I definitely find this idea appealing. Actually hearing the talks would indeed be quite educational. Cheers, Ronnie Berntsson On Aug 23, 2007, at 10:57 AM, Clemens Vonrhein wrote: Hi Eleanore, On Tue, Aug 21, 2007 at 12:56:40PM

Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-20 Thread Ronnie Berntsson
vel approach to phasing proteins: derivatization by short cryo- soaking with halides Z. Dauter, M. Dauter and K. R. Rajashankar Acta Cryst. (2000). D56, 232-237 Cheers, Ronnie Berntsson On Apr 20, 2007, at 6:26 AM, Tiancen Hu wrote: Dear all, Could any one recommend some heavy atoms used for cr