Hi Tiancen,
I can recommend using a Halide soak (bromide or iodine). You could
try to "exchange" part your salt with sodium bromide. Always use a
bad looking crystal for the first soak though to see that it doesn't
get damaged by the soak.
See for further reference:
Novel approach to phasing proteins: derivatization by short cryo-
soaking with halides
Z. Dauter, M. Dauter and K. R. Rajashankar
Acta Cryst. (2000). D56, 232-237
Cheers,
Ronnie Berntsson
On Apr 20, 2007, at 6:26 AM, Tiancen Hu wrote:
Dear all,
Could any one recommend some heavy atoms used for crystals grown in
0.1M tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M
ammonium sulfate? I read from Hampton user guide of heavy atom kit
that "high salt concentrations are not the ideal medium for heavy
atom reactions with macromolecules", so is there any type of heavy
atom we should avoid using? We do not have any experience in
preparing heavy atom derivative, so any suggestions, experience or
references will be greatly appreciated.
Thanks in advance!
Tiancen Hu
Shanghai Institute of Materia Medica
Ronnie Berntsson
----------
Ph.D. Student
Department of Biochemistry
Groningen Biomolecular Sciences and Biotechnology Institute
& Zernike Institute for Advanced Materials
University of Groningen
Nijenborgh 4, 9747 AG
Groningen, The Netherlands
telephone: +31 50 363 4195
telefax: +31 50 363 4165
e-mail: [EMAIL PROTECTED]
homepage: http://www.rug.nl/gbb/research/researchgroups/enzymology/index
