Hi Tim, Lactococcus lactis can also produce SeMet proteins, see: Berntsson et al. Selenomethionine incorporation in proteins expressed in Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7
Cheers, Ronnie On Mar 23, 2010, at 14:40, Tim Gruene wrote: > Dear all, > > I already received a couple of replies. > > * mammalian cells: > http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf > mentions several mammalian cell lines, including CHO, HEK239, and COS. > Apparently, Lec8 have been used, too. > * Yeast > * stable S2 insect cells > * baculovirus expression system > * in addition to E.coli, P. aeruginosa was also mentioned. > > Thanks to everyone who replied. > > Tim > > > On Tue, Mar 23, 2010 at 01:25:56PM +0100, Tim Gruene wrote: >> Dear all, >> >> since I am currenty preparing a lecture on crystallography I am wondering >> about >> the status quo of the production of SeMet proteins. >> In 2003, if I remember correctly, it was possible to express SeMet proteins >> in >> E.coli and insect cells. >> >> Has this been extended to other systems, and if so, which ones? >> >> Thanks a lot, >> Tim >> >> -- >> -- >> Tim Gruene >> Institut fuer anorganische Chemie >> Tammannstr. 4 >> D-37077 Goettingen >> >> GPG Key ID = A46BEE1A >> > > > > -- > -- > Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A >
