Re: [ccp4bb] Connecting DNA between asymmetric units?

be bigger to accommodate the 60 bases long DNA). I’ll think about it some more.. Best wishes, Ronnie -- Ronnie Berntsson, PhD Chair of the Young Academy of Sweden Department of Medical Biochemistry and Biophysics, Umeå University 90187 Umeå, Sweden e-mail: ronnie.bernts...@umu.se

[ccp4bb] Connecting DNA between asymmetric units?

know if any clarification is needed. Best wishes, Ronnie -- Ronnie Berntsson, PhD Chair of the Young Academy of Sweden Department of Medical Biochemistry and Biophysics, Umeå University 90187 Umeå, Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone:

[ccp4bb] Job opening at ModeX

ModeX Therapeutics is seeking a highlyskilled and motivated scientist to support biologics production, characterization,and optimization for developing innovative biotherapeutics. This is an excitingopportunity to be part of a cross-functional team and contribute to thedevelopment of next-gener

[ccp4bb] Senior Scientist in Antibody Engineering position available

work environment and capable to work both independently andcollaboratively within project teams. To apply, please send cover letter and CVto ronnie@modextx.com and care...@modextx.com To unsubscribe from the CCP4BB

Re: [ccp4bb] open review?

. I’ve used this a couple of times to look at grant proposals within my field as a learning experience. Best wishes, Ronnie Sent from my iPhone > On 23 Jun 2022, at 03:09, James Holton wrote: > > Greetings all, > > I'd like to ask a question that I expect might ge

[ccp4bb] Job opening

biochemistry and evaluating projects within Seqirus and external opportunities. This position will lead the vaccine antigen | | | | Best,Ronnie  To unsubscribe from the CCP4BB list, click the following link: https

[ccp4bb] How to make an isopeptide bond?

either, so I was hoping that someone here might know of an (preferably easy) way to do this? Best wishes, Ronnie -- Ronnie Berntsson, PhD Member of the Young Academy of Sweden Chair of the Swedish Society for Biochemistry, Biophysics and Molecular biology Department of Medical

[ccp4bb] Postdoc fellowship available to study assembly and structure of G+ T4SS at Umeå University, Sweden

with previous skill in molecular biology, biochemistry and cryo-EM. For more info about the project, qualifications expected and how to apply (deadline 10th of February, 2021), please see here: https://www.biostruct.umu.se/postdoc-position-available-in-the-berntsson-lab/ Best wishes, Ronnie

[ccp4bb] Postdoctoral fellowship available for studies of adhesion in bacterial conjugation

Dear all, A postdoctoral fellowship is available in my group at Umeå University, Sweden (https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/). Our lab studies bacterial conjugation via Type 4 Secretion Systems, with a special focus on gram-positive bacteria. The open position

[ccp4bb] PhD studentship at Umeå University working on Type 4 Secretion Systems

ronnie.bernts...@umu.se Best wishes, Ronnie -- Ronnie Berntsson, PhD Associate Senior Lecturer Wallenberg Molecular Medicine Fellow Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone:

[ccp4bb] Postdoctoral fellowships to study Type 4 Secretion System at Umeå University, Sweden

r more information about the research or other details, do not hesitate to contact Ronnie Berntsson. Email: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> Webpage: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/ -- Ronnie Berntsson, PhD Associate Senior Lecture

[ccp4bb] Job opening

A career opportunity if you want to pivot your structure Biology experience for something new.Check out this job: Protein Antibody Engineer https://www.linkedin.com/jobs/view/1031138315 To unsubscribe from the CCP4BB list,

[ccp4bb] 2 postdoctoral fellowships available to study Type 4 Secretion Systems at Umeå University, Sweden

Dear colleagues, I have two postdoctoral fellowships available in my group. Please see below for the details and feel free to spread it to anyone you think might be interested and suitable for the positions. Best wishes, Ronnie Postdoctoral fellowships to study Type 4 Secretion System The

[ccp4bb] PhD position available in Structural Biology of Type 4 Secretion Systems at Umeå University, Sweden

.se>. Applications must be sent in via the online recruitment system, with a deadline at 7th of December. Regards, -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronn

[ccp4bb] Postdoc position in membran protein structural biology

Please also see our websites: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/ http://www.medchem.umu.se/english/research/principal-investigators/berntsson-ronnie/ With best regards, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Bioc

[ccp4bb] PhD student position available at Umeå University

the online system and not directly to me. Best wishes, -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@umu.se<mailto:ronnie.bernts...@umu.se> phone: +46 90 7865235

[ccp4bb] Structural biologist position at Sanofi

Description Sanofi Genzyme focuses on developing specialty treatments for debilitating diseases that are often difficult to diagnose and treat, providing hope to patients and their families.Genzyme Corporation (a Sanofi company) and its U.S. affiliates are Equal Opportunity and Affirmative Actio

[ccp4bb] Choice of stereomicroscope

. Do any of you have other suggestions regarding the possibility of adding UV to a stereomicroscope? Suggestions and thoughts are more than welcome! Thanks, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå

[ccp4bb] Post-doctoral position at Umeå University, Sweden

Dear all, A post-doctoral position is available in the group of Dr. Ronnie Berntsson at the Department of Medical Biochemistry and Biophysics, Umeå University, Sweden. The project will concern structural and functional characterisation of the adhesion proteins involved in type 4 secretions

[ccp4bb] Postdoctoral position in the Stenmark lab at Stockholm University

Dear all, I’m posting this postdoctoral ad on behalf of Associate Professor Pål Stenmark. Please do not respond to me, but to struct...@dbb.su.se <mailto:struct...@dbb.su.se> Best wishes, Ronnie Postdoctoral position in structural biology at Stockholm University The Stenmark group h

[ccp4bb] Contract Scientist Opening at Sanofi, MA, US

biochemistry with 8+ years of experience in protein expression, purification, crystallization with strong protein chemistry skills or a recent Ph.D in protein crystallography with <1 year of experience post degree.   Please send CV to ronnie@genzyme.com

Re: [ccp4bb] Off topic Chromatography systems

Agilent also has some decent systems. Cheers, Ronnie -- Ronnie Berntsson, PhD PostDoctoral Fellow Department of Biochemistry and Biophysics Arrhenius Laboratories for Natural Sciences Stockholm University 10691 Stockholm Sweden On 18 Feb 2014, at 17:15 , Scott Pegan wrote: >

[ccp4bb] Postdoctoral position in structural biology at Stockholm University

I'm posting this on behalf of Prof. Pål Stenmark. Please direct all correspondence regarding this position to the email specified below (struct...@dbb.su.se). Cheers, Ronnie Berntsson The Stenmark group study novel cancer targets involved within human nucleotide metabolism. This proje

[ccp4bb] fluorescent pedal

I am trying to find out where I can get the fluorescent material (just a small flat piece) I can glue to the tip of a pin for aligning the X-ray beam of our home source. Does anyone know? Thanks in advance! Ronnie

[ccp4bb] no link_id

I am asking this question on behalf of a friend: -Thanks! Hi All - I am working at small biotech.  We have minimal budget for software, only CCP4 and Coot.  We have a ligand that binds covalently to cysteine.  I define the parameter file using Prodrg in CCP4 to get drug.cif file and then make a l

[ccp4bb] 3D printing structures?

representation for example? Thanks! Ronnie

[ccp4bb] Job opening at Genzyme/Sanofi

Title: Scientist Staff I-32530 Description: Genzyme has pioneered the development and delivery of transformative therapies for patients affected by rare and debilitating diseases for over 30 years. We accomplish our goals through world-class research, collaboration with the global patient commun

Re: [ccp4bb] Coot 0.7 Saving Dialog opens behind main window

Yes, it's still happening on Mountain Lion. Cheers, Ronnie -- Dr. Ronnie Berntsson PostDoctoral Fellow Department of Biochemistry and Biophysics Arrhenius Laboratories for Natural Sciences Stockholm University 10691 Stockholm Sweden On Oct 30, 2012, at 16:51 , Antony Oliver

Re: [ccp4bb] Checking in dry shippers?

well for the past years, no hiccups. Cheers, Ronnie Berntsson On Apr 18, 2012, at 10:06 , herman.schreu...@sanofi.com wrote: > (very last spam) > At a certain moment, we were flying once or twice a month, so by then I > thought they would know dewars... > It is extremely depending o

[ccp4bb]

http://tdnsoftware.by.ru/romio1.html";>http://tdnsoftware.by.ru/romio1.html

[ccp4bb] disulfide bond question

m not sure how stable it is, or how energetically unfavorable it might be. thanks so much for your input! Ronnie

Re: [ccp4bb] summary: off topic: advice for crystallography workstation/server

ptops. This can be done just by using ssh and running the normal os x on the machine, and not using vnc (or similar). I believe this might work quite well in our lab, but I'll need to give some thought. Again, thanks for your suggestions. Cheers, Ronnie On Dec 15, 2010, at 20:07, Bosc

[ccp4bb] off topic: advice for crystallography workstation/server

would very much appreciate your advice or personal experiences regarding this matter. I know that I could probably get a cheaper computer if I went for a pc with linux, but I have more experience with OS X, and would therefore want to stay with it. Thank you in advance, Ronnie

Re: [ccp4bb] High Rmerge with thin frames

and would like to maximize the potential data quality from our weak diffracting crystals. Any input on this would be greatly appreciated! Cheers, Ronnie Berntsson On Nov 5, 2010, at 16:16, Anastassis Perrakis wrote: > three additional points: > > 1. > >> OTOH, if "The di

[ccp4bb] chainsaw with multiple chains

If i want to use chainsaw to prep a pdb file that contains two chains of different sequences, how do I format the alignment .pir file to include the alignments for both chains? thanks for in advance for your help! Ronnie

Re: [ccp4bb] update on SeMet production

Hi Tim, Lactococcus lactis can also produce SeMet proteins, see: Berntsson et al. Selenomethionine incorporation in proteins expressed in Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7 Cheers, Ronnie On Mar 23, 2010, at 14:40, Tim Gruene wrote: > Dear all, > >

Re: [ccp4bb] off topic: multiple structural sequence alignment

Dear all, Thanks a lot for all the replies and suggestions. I'm currently busy trying out a few of the options, and I'll try to post a brief summary after I've finished the analysis. Cheers, Ronnie On Jan 15, 2010, at 09:12, kuett...@bbz.uni-leipzig.de wrote: > Dear Ron

[ccp4bb] off topic: multiple structural sequence alignment

very grateful. Cheers, Ronnie Berntsson

Re: [ccp4bb] Minimum hardware requirement of Mac OSX for CCP4

ing machine for crystallography, and even though it is a couple of years old there is basically nothing it still working fine with all software. So a new iMac will work just fine. Cheers, Ronnie Berntsson On Oct 27, 2009, at 13:06, J. Attarataya wrote: Dear Sir, I am a new postgraduate fro

[ccp4bb] postdoc position at Genzyme Corp

Dear all, I want to bring your attention to a crystallographer postdoc opening at Genzyme Corporation. Please see the job description below and for information on how to apply. Thanks very much! Ronnie Post Doctoral Associate Genzyme Corporation, ranked as one of the foremost

Re: [ccp4bb] How to express a >95KD FAD protein

ophys Acta (2003) 97-108, Geertsma and Poolman. Nat Methods (2007) 705-7, Kunji et al. Curr Opin Biotechnol (2005) 546-51. Cheers, Ronnie Berntsson On Jun 29, 2009, at 23:54, Yong, Wei wrote: Dear all, I know that there are a lot of experts having experience in expressing a big protein in E

Re: [ccp4bb] XDS and ESRF ID23-1 images

, Ronnie On Mar 27, 2009, at 14:46, Miguel Ortiz Lombardia wrote: Dear all, Not a CCP4 question. We seem unable to process with XDS a set of images collected at ESRF ID23-1. Images can be open and processed by other software (adxv, mosflm...) They have appropriate read permissions and so has

Re: [ccp4bb] Twin refinement not working in CCP4 6.1.1

I have the same problem when running the latest ccp4 (6.1.1 on os x, updated 1 hour ago via fink). Only way I managed to circumvent it is to add the TWIN keyword in the command file as well.. Cheers, Ronnie Berntsson On Mar 2, 2009, at 16:06, Carr, SB (Stephen) wrote: Dear CCP4BB, I have

[ccp4bb] 1-butyl-2-methyl imidazolium chloride

Can anyone tell me where to purchase 1-butyl-2-methyl imidazolium chloride? I'd like to try it as an ionic liquid as suggested on the Hampton website "Tips from RAMC". Thanks so much! Ronnie

Re: [ccp4bb] dry shipper on airplaine

poses any trouble, they seem to be used to dewars. Cheers, Ronnie On May 27, 2008, at 2:28 PM, Clemens Grimm wrote: Dear all, I wonder if it would be still/again possible to check in a dry shipper for a flight inside Europe. What is your experience? Cheers, Clemens

Re: [ccp4bb] font size CCP4i

XQuarts X11 (http://xquartz.macosforge.org/trac/wiki). Newest version of X11.pkg is 2.2.1, and it should fix the font issue in CCP4i. Cheers, Ronnie On Fri, 23 May 2008 14:47:31 +0200 Kristof Van Hecke <[EMAIL PROTECTED]> wrote: Dear all, I recently installed the CCP4 Program Suite

Re: [ccp4bb] arp/warp and refmac problem on os x

Thanks for a very quick answer, it indeed solved the problem. Cheers, Ronnie On Apr 24, 2008, at 4:55 PM, Chris Richardson wrote: On 24 Apr 2008, at 3:38 PM, Ronnie Berntsson wrote: I've got a rather weird problem when trying to run arp/warp. When trying to run arp/warp (latest ve

[ccp4bb] arp/warp and refmac problem on os x

d ccp4 and refmac via fink. All other CCP4 programs work fine and Refmac runs normally as well, it's only when it's called from arp/warp that something goes wrong.. All ideas to fix this problem are most welcome. Cheers, Ronnie Berntsson

Re: [ccp4bb] crashing-out protein eluted from Nickel column

Hi, In addition to the tips already suggested, you could also try to elute your protein with histidine.. I've got a protein that crashes out with imidazole as well, and I have succesfully used 200mM histidine for elution (using either KPi or MES buffer in my case). Cheers, Ronnie O

Re: [ccp4bb] microsoft 3-button wheel mouse with OS X 10.5

to the trick and save you quite some extra mouse clicks. Cheers, Ronnie On Jan 23, 2008, at 4:24 PM, William Scott wrote: Yes, thanks, that does it for the Terminal.app, but not for any of the rest. It would be great to have such a feature globally. mb1pja wrote: Dear Bill William Scott

[ccp4bb] glycosylation sites

I was asked this question by a colleague. Has anyone looked into where glycosylation occurs most frequently on a protein- loops, alpha-helices or beta-strands? Thanks for your input! Best, Ronnie

Re: [ccp4bb] problem on protein precipitation

100kDa. High detergent concentrations are usually not very good for the protein in the long run. Cheers, Ronnie On Jan 24, 2008, at 9:12 AM, M T wrote: 2008/1/23, Zheng, Lei <[EMAIL PROTECTED]>: Hi ccp4ers, Sorry for this out-topic question: Recently we have a membrane protein exp

[ccp4bb] Postdoc position at Groningen University

to apply, please email a current CV including a brief description of your research interests and accomplishments, and the names and addresses of two referees to Bert Poolman ([EMAIL PROTECTED]) or Dirk Slotboom ([EMAIL PROTECTED]). Please do not respond to me. Thank you. Ronnie Berntsson

Re: [ccp4bb] Apple OS 10.5 and X11

f the programs. I'll spend some time thoroughly testing the build later this week. Cheers, Ronnie On Nov 5, 2007, at 7:03 PM, R.M. Garavito wrote: After Bill Scott's and Juergen Bosch's comments about upgrading to Leopard 10.5, it has been quiet. As I am upgrading some "

Re: [ccp4bb] paper from mol microbiol

I have recieved the paper. Thanks a lot, Ronnie - Ronnie Berntsson Ph.D. Student Biochemistry-Enzymology University of Groningen Nijenborgh 4, 9747 AG Groningen, The Netherlands telephone: +31 50 363 4195 telefax: +31 50 363 4165 e-mail: [EMAIL PROTECTED] homepage: http

[ccp4bb] paper from mol microbiol

1997 and onwards, and unfortunately the librarys printed copy is somewhat difficult to localize... If someone has it lying around I'd be very grateful! With regards, Ronnie Berntsson -- Ph.D. Student Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology

Re: [ccp4bb] SciVee - YouTube for scientist

Hi all, Great idea! As being reasonably new to the field, I would definitely welcome those videos. Not that I don't read Acta D papers, but it is always nice to hear the talks directly, and would certainly be quite educational. Cheers, Ronnie Berntsson -- Ph.D. St

Re: [ccp4bb] SciVee - YouTube for scientist

Hi all, Great idea! As being relatively new to the field, I definitely find this idea appealing. Actually hearing the talks would indeed be quite educational. Cheers, Ronnie Berntsson On Aug 23, 2007, at 10:57 AM, Clemens Vonrhein wrote: Hi Eleanore, On Tue, Aug 21, 2007 at 12:56:40PM

Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

vel approach to phasing proteins: derivatization by short cryo- soaking with halides Z. Dauter, M. Dauter and K. R. Rajashankar Acta Cryst. (2000). D56, 232-237 Cheers, Ronnie Berntsson On Apr 20, 2007, at 6:26 AM, Tiancen Hu wrote: Dear all, Could any one recommend some heavy atoms used for cr