[ccp4bb] PhD studentship available at Newcastle and Monash University, Australia - fully funded (UK only)

Dear colleagues I’ve got a fully funded PhD studentship (UK only) in my lab and in collaboration with Prof Dena Lyras at Monash University, and I’m reaching out to ask for your help in spreading the word. It's focused on S-layers variants and is a multidisciplinary project, with 2 years at Newca

[ccp4bb] 2 fully funded PhD studentships in Newcastle University

Dear colleagues Just wanted to highlight 2 fully funded PhD studentships in my lab with strong structural biology focus - protein crystallography, cryoEM, cryoET and combinatorial approaches, combined with biochemistry and microbiology to study antibiotic-resistant pathogen C. difficile. Would

[ccp4bb] Structural biology+molecular simulations PhD studentship at Newcastle University

Dear all We have a BBSRC-funded PhD studentship available and would appreciate if you can circulate to your institutions and potential candidates. https://www.findaphd.com/phds/project/piercing-the-armour-c-difficile-s-layer-permeability/?p150321 The project builds on our recent work on C. diff

Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure

Hi Alan We got one a few years back, from Fisher, although I think Thermo are the manufacturer's: Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA FRCR4504W They also arranged for a heavy metal shelf to hold the AKTA as the normal shelves won't hold the weight. We only have

Re: [ccp4bb] CCP4BB vs COVID19

Same for Newcastle University, particularly our Faculty of Medical Sciences but colleagues and labs from across the University. There are also several efforts to get scientists to help: http://crowdfightcovid19.org/volunteers Thanks to all those trying to help! Paula On 21 Mar 2020, at 11:5

[ccp4bb] Propeptide cleavage for complex assembly

Dear colleagues We have been working on a protein that is produced as a pro-peptide, cleaved internally and reassembled into a complex. The interacting regions are the new C and N-termini at the cleavage site, so no rearrangement or loss of part of the protein is involved. The resulting interac

Re: [ccp4bb] challenges in structural biology

Very well said, Susan! I totally agree and thank you for saying it so clearly and eloquently! Best wishes Paula === Dr Paula S. Salgado Senior Lecturer in Macromolecular Crystallography Institute for Cell and Molecular Biosciences Faculty of Medical Sciences 3

Re: [ccp4bb] 40th CCP4 anniversary competition - 10 Golden Tickets deadline 18th June!

)191 208 7432 Fax: +44 (0)191 208 7424 Email: paula.salg...@ncl.ac.uk From: CCP4 bulletin board on behalf of Paula Salgado Sent: 03 June 2019 15:47:28 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] 40th CCP4 anniversary competition - 10 Golden Tickets deadline 18th

Re: [ccp4bb] Se-Met and Se-Cys double labelling

We have successfully used non-auxotrophic strains for incorporation of SeMet and SeCys, with an incorporation level of >80% and no effects on yield or solubility. Details can be found here: http://scripts.iucr.org/cgi-bin/paper?S0907444910042022 It's a straight-forward protocol and structure

Re: [ccp4bb] Glass etching of structure

I recently used Luminorum and was very happy with the result - and they managed to do it last minute as well! http://www.luminorum.com/shop/ Paula === Dr Paula S. Salgado Lecturer in Macromolecular Crystallography Institute for Cell and Molecular Biosciences

[ccp4bb] OT: PhD position available

Dear colleagues I have an MRC-funded PhD position currently available in my lab to study proteins involved in sporulation of the human pathogen Clostridium difficile (details below). I would appreciate if you can pass the information to potential students and/or advertise it in your institution

Re: [ccp4bb] ITC with unfolded proteins

Dear Anita One alternative method to determine the thermodynamics and potentially discern the folding energy changes from the interaction driven ones would be NMR. Advantages over ITC experiments include: determine if the interaction drives foldness, estimate associated thermodynamics and, if

[ccp4bb] off topic: Science is Vital - campaign for science funding in UK

Dear all This is mostly relevant for those of you in the UK, so I apologise for the off tpopic nature of the email. However, I think this is an important issue for the CCP4 community as a whole as well, since UK funding changes will affect how CCP4 is funded and the continued support we all get

Re: [ccp4bb] SeCys usage for SAD

Dear Linda As we described, we used an adapted minimal media to introduce SeCys into our recombinantly expressed protein. As a SeCys source, we used seleno-cistine (provided by Sigma) which is reduced to seleno-cysteine and incorporated into the proteins. It works really well and incorporation

[ccp4bb] Research Associate position at Imperial College London - on behalf of Prof Fairweather

Dear all, Please find below an ad for a post-doctoral researcher vacancy at Imperial, posted hereon behalf of Prof Fairweather. Please reply directly to him or the email refered in the ad. Best regards Paula *Imperial College London, Division of Cell & Molecular Biology, Faculty of Natural Sc

Re: [ccp4bb] Fwd: HR3699, Research Works Act

May I also suggest reading these: https://intechweb.wordpress.com/2012/01/25/selected-reading-on-research-works-act-why-you-should-care/ https://intechweb.wordpress.com/2012/02/16/open-access-on-a-string-cut-it-and-it-will-grow-back/ Can non-US based scientists sign the petition, btw? There ar

[ccp4bb] PhD studentship opportunity in Newcastle University

gards Dr Paula Salgado

Re: [ccp4bb] Se-Met Protein Purification

Dear Abhilash You could try an alternative labelling option: if your protein has cysteines, try labelling those with selenium. We succeeded in doing this using non-auxotrophic strains, meaning you deviate less from your native expression protocol and are therefore more likely to obtain well folded

Re: [ccp4bb] Mg2+ or water

Another option would be to collect data at the Mn K edge (1.89A) - at this wavelength Mg has a weak anomalous signal that you should still be able to detect. I've used Mn K edge to successfully distinguish between Mg, Mn and Ca ions as well as identify them as ions and not waters by looking at pea

Re: [ccp4bb] Cys auxotroph

the future. Best regards Paula On 20 October 2010 12:00, Paula Salgado wrote: > We are still waiting for proofs, so it might be a few weeks before its > published. If anyone is interested, please email me requesting a preview > version. > > Paula > > > PS: Francois, we did

Re: [ccp4bb] Low B-factors

protein at 1.7A... Must admit I never had such nice structures before, so I was being extra careful. Paula On 23 November 2010 19:27, Ethan Merritt wrote: > On Tuesday, November 23, 2010, Paula Salgado wrote: > > Dear all > > > > I'm refining a 33kDa protein model and I

[ccp4bb] Low B-factors

n chain A 2352 Average B value for main chain A 20.651 Average B value for side chain A 21.815 Average B value for whole chain A 21.199 Any help and advice is most appreciated. Best regards Paula

Re: [ccp4bb] Cys auxotroph

We are still waiting for proofs, so it might be a few weeks before its published. If anyone is interested, please email me requesting a preview version. Paula PS: Francois, we did cite and used your work as starting point. On 20 October 2010 10:39, francois@cbs.cnrs.fr wrote: > Hi Paula >

Re: [ccp4bb] Cys auxotroph

I would also try non-auxotrophic strains. We have recently had successful double SeCys + SeMet using BL21. Paper has just been accepted in Acta D, should be published soon. Paula On 18 October 2010 16:05, Yogesh Gupta wrote: > Does anyone know a commercial source of Cys-auxotroph strain of E.

Re: [ccp4bb] problem with Coot:findwaters in ccp4i

i from. Does > that yield a 'command not found'? > > Tim > > -- > Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > > > On Tue, 19 Jan 2010, Paula Salgado wrote: > > > Hi everyo

[ccp4bb] problem with Coot:findwaters in ccp4i

*#CCP4I MESSAGE Task failed* I checked and the Coot/bin directory exists and it contains a "findwaters" executable file. I should add that doing "find waters" within Coot works fine. Any ideas? All help welcomed. Thanks! Paula =

Re: [ccp4bb] FW: pdb-l: Retraction of 12 Structures

2009/12/11 Dale Tronrud > > > Paula Salgado wrote: > > > > Actually, I don't think that should be any consolation at all... As > > scientists, from whatever field, we should be appalled by this kind of > > mischief from anyone that calls themselves scie

Re: [ccp4bb] FW: pdb-l: Retraction of 12 Structures

es an appalling image of science and scientists. And of course, is unethical and wrong... Today is a sad moment for crystallography and science. = Dr Paula Salgado Division of Molecular Biosciences Department of Life Sciences Facul

Re: [ccp4bb] To get the crystal faster...

. If you still have crystals, run one on a gel and if the MW seems different, you can try to investigate further. It might mean getting a new, shorter, more "crystallizable" construct. Good luck Paula Salgado 2009/10/11 james09 pruza > Dear crystallographers, > > Sorry for the n