For those interested in our paper using non-auxotroph strains for SE-Cys labelling, it's now published:
http://journals.iucr.org/d/issues/2011/01/00/dz5216/index.html <http://journals.iucr.org/d/issues/2011/01/00/dz5216/index.html>Please cite this reference if you use our method in the future. Best regards Paula On 20 October 2010 12:00, Paula Salgado <p.salg...@imperial.ac.uk> wrote: > We are still waiting for proofs, so it might be a few weeks before its > published. If anyone is interested, please email me requesting a preview > version. > > Paula > > > PS: Francois, we did cite and used your work as starting point. > > On 20 October 2010 10:39, francois....@cbs.cnrs.fr < > francois....@cbs.cnrs.fr> wrote: > >> Hi Paula >> >> Also our publication: http://www.ncbi.nlm.nih.gov/pubmed/14604526 >> We can provide you the Cys-auxotroph strain of E. coli >> >> Francois >> >> >> =============== Francois Hoh, PhD ================ >> CBS Phone : (33) 4 67 41 77 06 >> 29 rue de Navacelles Fax : (33) 4 67 41 79 13 >> 34090 MONTPELLIER francois....@cbs.cnrs.fr >> France >> www.cbs.cnrs.fr >> >> >> >> > I would also try non-auxotrophic strains. We have recently had >> successful >> > double SeCys + SeMet using BL21. Paper has just been accepted in Acta D, >> > should be published soon. >> > >> > Paula >> > >> > On 18 October 2010 16:05, Yogesh Gupta <yogesh.gupt...@gmail.com> >> wrote: >> > >> >> Does anyone know a commercial source of Cys-auxotroph strain of E. >> coli >> >> to >> >> prepare a doubly (Se-Cys + Se-Met) labeled protein? I would appreciate >> >> if >> >> you can share your experience if you have labeled your protein in this >> >> way >> >> to improve Se signal for phasing. >> >> >> >> Thanks, >> >> -Yogesh >> >> >> >> -- >> >> -------------------------------------------------- >> >> Yogesh K. Gupta, Ph.D. >> >> Dept of Structural & Chemical Biology >> >> Mount Sinai School of Medicine >> >> Icahn Medical Institute >> >> 1 Gustave L. Levy Place, Box 1677 >> >> New York, NY, USA 10029 >> >> >> >> Tel:+1 212-659-8639 >> >> -------------------------------------------------- >> >> >> > >> >> >> >> >> >> >> >> >
