I tend to like considering below 10microns as a micro-focus, below the micron
as a sub-micron beamline. I don't know if any nano-focus, sort to say. XFELs
are dealing with 100 nm, without the wings, which is really sub-micron to me.
Regards, Leo
-
Leonard Chavas
-
Synchrotron SOLEIL
PROXIMA-1
L
Users had issues, back at SOLEIL in France, with FedEx, DHL, UPS, French
post... at many levels. I guess it just depends on luck, or bad luck in the
present case. The worst case encountered so far though was Dewars lost in
transit for a couple of weeks. Not to mention countries where import taxe
Dear Chandra
SLS and ourselves (SOLEIL) are dealing rather often with large unit cells and
fancy crystal orientations. No great secret, and things have already been
mentioned: combining three-axis goniometry, large area Pixel detector, low
background, small wedges and/or helical scans, Staranis
Dear all
I had one interesting comment today at the beamline, that I would like to share
with you for your advice on how to answer this...
Is it better to have no diffraction from a crystal you shoot, or to have no
crystal in the loop?
Hum, difficult one...
Cheers, leo
-
Leonard Chavas
-
Sy
Dear all
please consider the following post-doctoral position available at the
Synchrotron SOLEIL in France. Would you or colleagues be interested, I would
greatly appreciate if you could forward this message to them.
Cheers, leo
Postdoctoral position in life sciences (24 months)
Synchrotron
Hi Frank
on the same lines: do we keep our crystals after frying them? Or do molecular
biologists keep their agarose gels? Hummm... evolution and technologies do
progress.
Yet, I would support keeping images, just as you may (but really will most
likely never) want to re-process those with new
Dear Gerard
I should certainly be the one to blame, as I was the one spotting this
behaviour to Stefan. Telling this, it could indeed be a problem with the data
set, yet submitting the original mtz to ContaMiner provided with the strangely
hoped and expected ContaMiner negative result. I made f
Dear all,
to join Pierre's comments on what 'strange' things happen at the beamlines...
yet not too strange for (too) many people: huge screening of salt crystals,
complete data collection of dramatically low resolution data, full power
coupled with 360Deg data collection etc. etc. etc. We do
d.cha...@synchrotron-soleil.fr
-
> On 14 Jul 2017, at 14:07, CHAVAS Leonard
> wrote:
>
> Just to comment on what Graeme just introduced. We (and I know we are not the
> first ones and not the only ones) are pushing our user community towards this
> procedure as a standard: lo
Attenuation... cut the beam with primary slits! We do not use attenuators, only
for getting very very low when performing energy scans. Else, cutting the flux
at the source is somehow much more reliable. Not mentioning scattering coming
from the attenuators as well...
Cheers, leo
-
Leonard Cha
Just to comment on what Graeme just introduced. We (and I know we are not the
first ones and not the only ones) are pushing our user community towards this
procedure as a standard: lowering the transmission (less juicy, yet...) and
getting few data with various chi. It does help greatly in getti
Dear Kumar
you said 'shot from other conditions': we do not know then if you shot these
crystals or not.
Else:
- DNA should give you an X pattern, most of the time.
- protein should give you at least low resolution pattern, or faint rings if it
is powder pattern like
- salt should give you dif
Do you have continuous chains all the way through? Any possibility of domain
swapping?
leo
-
Leonard Chavas
-
Synchrotron SOLEIL
Proxima-I
L'Orme des Merisiers
Saint-Aubin - BP 48
91192 Gif-sur-Yvette Cedex
France
-
Phone: +33 169 359 746
Mobile: +33 644 321 614
E-mail: leonard.cha...@synchro
Could we write to them as a community? As the 'CCP4 Peer Reviewing Organisation
for Structural Biology'?
Great job indeed!
Cheers, leo
-
Leonard Chavas
-
Synchrotron SOLEIL
Proxima-I
L'Orme des Merisiers
Saint-Aubin - BP 48
91192 Gif-sur-Yvette Cedex
France
-
Phone: +33 169 359 746
Mobile: +
Dear all
something I am not completely aware of: can the ESRF users decide on having
their data destroyed from the ESRF servers? One way to go I would think could
be proper would be to announce getting open access for all the data on the
servers or wherever after 3 years (or few months for what
, leo
> On 21 Mar 2016, at 15:09, Manjula Ramu wrote:
>
> I had no reducing agent in my protein buffer as wel as in crystal condition.
> I have only sodium citrate in the condition.
>
> On Mar 21, 2016 6:54 PM, "CHAVAS Leonard"
> wrote:
> DTT perhaps?
>
&g
DTT perhaps?
Cheers, leo
> On 21 Mar 2016, at 14:04, Pedro Matias wrote:
>
> Does it make sense to consider 2 extra S-atoms bridging the 2 cysteines? What
> would be the bond distances? What is the meaning of the red e.d. in the 2nd
> figure?
>
> Às 12:44 de 21/03/2016, Eleanor Dodson escrev
rting P1 for
> a crystal system consisting of higher symmetry?
>
> Cheers,
> Quyen
>
>> On Jan 29, 2016, at 4:27 PM, CHAVAS Leonard
>> wrote:
>>
>> Sorry if I don't buy it. Just personal feeling. I don't see how someone
>> capable of n
rapher would simply press a few buttons to generate a model
> with auto build?
> But I have a feeling those couple of buttons might not be so obvious to
> people in other fields.
>
> Quyen
>
>> On Jan 29, 2016, at 3:16 PM, CHAVAS Leonard
>> wrote:
&g
Not sure I fully understand. Are we really talking about non-crystallographer
scientists, often willing to understand how a biologically meaningful molecule
/ entity looks like? Are these non-crystallographers really interested in
crystal packing issues? Is it so much difficult to press on a cou
Hi Ivan
this will not be an answer to your question, but did you consider not
concentrating 'too much' your sample? It happened few times to me that the
protein was precipitating when concentrating for SEC because of the presence of
other impurities. Trying the good old AS precipitation helped
Dear Dialing
are you talking about the 'creating date' or the 'modified date'?
Leo
> On Jan 6, 2015, at 2:55 AM, Dialing Pretty
> <03f1d08ed29c-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Dear All,
>
> When I run Refmac, it would produce a refined PDB file and mtz file. My
> question is, if
Dear Jeorge
just as a curiosity:
1- do you see a continuous electron density for the ligand, before and after
performing the refinement?
2- do you have only one copy of the molecule(s), and if not, do you see the
same behaviour for all the ligands?
3- what are the B-factor values for the ligand?
Dear Jeorge
are the water molecules without electron density properly coordinated? These
could be ghosts... Additionally, did you solve the structure by molecular
replacement? If so, in your search model, do you see these water molecules at
the same / nearby location? Again, those could be mode
read the documentation? Probably somewhere it should be mentioned to have the
'source ' command in your bash file? I might have misunderstood your question
though.
Leo
> On 16 Nov 2014, at 07:30, 陈昂 wrote:
>
> Dear all:
>
> I have installed CCP4 suit on my computer. The OS IS fedora 18. Ever
Dear all
I came along a disturbing feature and I would appreciate to get your lights on
this.
After rebuilding our data server, plenty of access rights have been changed and
various folders have been ‘secured’ by restricting access to few users and
groups. The whole ccp4 suite has also been su
.
Cheers, Leo
On Dec 10, 2013, at 10:13 AM, Tim Gruene wrote:
> 'Will' is a fashionable word - but since even a Nobel prize was given
> based on promises never kept, who would be surprised...
>
> Best wishes from now,
> Tim
>
> On 12/10/2013 10:11 AM, CHAVAS Leonard w
Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much
less sample, and much more time available. But that's in few years.
Leo
On Dec 10, 2013, at 10:01 AM, Tim Gruene wrote:
> Dear Careina,
>
> you can also apply for beamtime at PETRA-III. You get away with the same
> s
Dear Jens, Careina
this is not really an *advantage*, but rather a *convenience*. You can still
use big crystals if you'd like to, but as they usually never survive more than
one shot (few femtosec), you'd need a lot of these bigger crystals to collect a
full data.
And yes, I would also highly
pan
(We accept recommendation or reference letter(s) by email)
Others :
- KEK is an equal-opportunity employer and encourages applications from women.
- This program will be evaluated in the third year.
-----------
Chavas Leonard M.G., Ph.D.
Assistant Pro
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