Indeed nothing is predictable. We can only assume things on what is *planed* and *promised*, and you can take my words on the fact that I will do my best to have it done properly and as close to the expectations as possible. But again, nobody can really predict the future. Finger crossed.
Cheers, Leo On Dec 10, 2013, at 10:13 AM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: > 'Will' is a fashionable word - but since even a Nobel prize was given > based on promises never kept, who would be surprised... > > Best wishes from now, > Tim > > On 12/10/2013 10:11 AM, CHAVAS Leonard wrote: >> Yes, Tim is right… for now… in few years, with the E-XFEL, we'll get to much >> less sample, and much more time available. But that's in few years. >> >> Leo >> >> On Dec 10, 2013, at 10:01 AM, Tim Gruene <t...@shelx.uni-ac.gwdg.de> wrote: >> >>> Dear Careina, >>> >>> you can also apply for beamtime at PETRA-III. You get away with the same >>> size crystals but only require a nano liter drop rather than a few ml of >>> your sample. And you probably get beamtime much quicker because all the >>> equipment is installed and collecting a data set takes very short time. >>> This was demonstrated at the ECM in Warwick this year, so no need for >>> FEL (at least for structure determination). >>> >>> Best, >>> Tim >>> >>> On 12/10/2013 04:36 AM, Jens Kaiser wrote: >>>> Careina, >>>> If your target is interesting enough, try to reproduce the small >>>> crystals in batch and apply for FELS time. Small crystals are actually >>>> an advantage there. >>>> >>>> Cheers, >>>> >>>> Jens >>>> >>>> >>>> On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote: >>>>> Hi all >>>>> >>>>> >>>>> Any advice on how to get bigger crystals from conditions that give >>>>> showers of tiny crystals? I am getting small pretty looking individual >>>>> crystals but they are too small and they don't seem to grow. In fact, >>>>> in some instances if left for a couple of days they actually dissolve. >>>>> I have fiddled around with mother liquor volume, protein concentration >>>>> as well as drop volume (I am using hanging drop method) but none seem >>>>> to make any difference and I always get the same tiny crystals. I >>>>> think I might try microseeding but I haven't tried that yet. >>>>> >>>>> >>>>> Any suggestions or tricks would be welcome >>>>> Careina. >>>> >>> >>> -- >>> Dr Tim Gruene >>> Institut fuer anorganische Chemie >>> Tammannstr. 4 >>> D-37077 Goettingen >>> >>> GPG Key ID = A46BEE1A >>> >> > > -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A >
