DTT perhaps? Cheers, leo
> On 21 Mar 2016, at 14:04, Pedro Matias <mat...@itqb.unl.pt> wrote: > > Does it make sense to consider 2 extra S-atoms bridging the 2 cysteines? What > would be the bond distances? What is the meaning of the red e.d. in the 2nd > figure? > > Às 12:44 de 21/03/2016, Eleanor Dodson escreveu: >> No idea, but it is a lovely map. >> >> Are you sure the residue is CYS? You could check the anom map to see if the >> S give a signal. >> Eleanor >> >> On 20 March 2016 at 04:36, Manjula Ramu <manjula....@gmail.com> wrote: >> Dear community, >> >> I have got a crystal structure of 16Kda protein, which was solved at 1.9 A. >> Protein crystallised in dimer form and I could observe a continuous extra >> density between cysteine of two chains. Distance between two intermolecular >> cysteine is 7.8 A. Please help me to interpret this extra density. Thanks >> in advance. >> I have attached the screen shots of the map. >> >> >> Thanks and Regards, >> Manjula R >> Research Scholar >> Department of Biophysics >> National Institute of Mental Health and Neurosciences >> Bengaluru-29, Karnataka >> INDIA >> E-mail: manjula....@gmail.com >> Mobile no:+91-9538553356 >> http://www.nimhans.kar.nic.in/ >> >> >> >> > > > -- > > Industry and Medicine Applied Crystallography > Macromolecular Crystallography Unit > ___________________________________ > Phones : (351-21) 446-9100 Ext. 1669 > (351-21) 446-9669 (direct) > Fax : (351-21) 441-1277 or 443-3644 > > email : > mat...@itqb.unl.pt > > > > http://www.itqb.unl.pt/research/biological-chemistry/industry-and-medicine-applied-crystallography > http://www.itqb.unl.pt/labs/macromolecular-crystallography-unit > > > Mailing address : > Instituto de Tecnologia Quimica e Biologica > Universidade Nova de Lisboa > Av. da República - EAN > 2780-157 Oeiras > PORTUGAL > > ITQB, a great choice for your PhD > > https://youtu.be/de6j-aaTWNQ > > > Master Programme in Biochemistry for Health > > https://youtu.be/UKstDCFjYI8 - Leonard Chavas - Synchrotron SOLEIL Proxima-I L'Orme des Merisiers Saint-Aubin - BP 48 91192 Gif-sur-Yvette Cedex France - Phone: +33 169 359 746 Mobile: +33 644 321 614 E-mail: leonard.cha...@synchrotron-soleil.fr -
