Dear all,
I need to make a figure which needs to consist of 2 aligned pdb files
and their electron density maps for the structures of two mutants
(with different space groups). I'm able to do this when there are two
structures that have the same space group. Could, you, please, help
me to
Hi,
Could you clarify your request a bit? Generally speaking if you allow the
ectodomain to have a membrane anchoring segment it will 'compromise'
solubility since the resulting protein most likely requires special
treatment (detergents) in order to be soluble. There's also the matter of
the se
Hi,if you add the TM segment, I would expect that the protein will then get
targeted to the membrane and hopefully inserted correctly. My guess is that
you will have to treat your protein as a membrane protein or a
membrane-anchored protein...which means prepare a membrane fraction, use
detergent a
may be diafiltration devices might work for you. the membranes are
kind of expensive but we had used this effectively to reduce the volume
of cell culture media where we had secreted proteins in large volumes
of culture media.
here is a link
http://www.spectrapor.com/lit/hfdial.pdf
padayatti
On
may be diafiltration devices might work for you. the membranes are
kind of expensive but we had used this effectively to reduce the volume
of cell culture media where we had secreted proteins in large volumes
of culture media.
here is a link
http://www.spectrapor.com/lit/hfdial.pdf
padayatti
On
If you want to just reduce the volume/concentrate proteins, you can use
Millipore's Stirred cell (high-output stirred cell, 2,000 ml capacity).
Zhongren Wu
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Xuan Yang
Sent: Thursday,
Dear All
I am working with a viral protein that contains an ectodomain, a TM segment and
a cytoplasmic region. I was wondering if there is a general strategy that
allows the addition of the transmembrane segment to the ectodomain without
compromising solubility and secretion.
Thanks
Dr. R. D
Lower the concentration did help a little bit. I didn't the 6 mg/ml setups,
but still got some skins.
You are right the skins are more likely to find in the condition of
PEGs...but also find in other conditions.
Lower the conc. of PEGs is a good advice, and I'll try it. Thanks.
Shu
On Sun, Sep 6,
This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration
Dear CCP4bbers,
Please comment on the stabilizing solution for seed stocks. If the crystal
is in 30% MPD and coming after 2-3 days, what should be the stabilizing
solution for seeding.
Thanks.
James
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