may be diafiltration devices might work for you. the membranes are kind of expensive but we had used this effectively to reduce the volume of cell culture media where we had secreted proteins in large volumes of culture media.
here is a link http://www.spectrapor.com/lit/hfdial.pdf padayatti On Sun, Sep 6, 2009 at 10:16 PM, Wu, Zhongren<z...@vitaerx.com> wrote: > If you want to just reduce the volume/concentrate proteins, you can use > Millipore’s Stirred cell (high-output stirred cell, 2,000 ml capacity). > > > > Zhongren Wu > > > > > > ________________________________ > > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Xuan > Yang > Sent: Thursday, September 03, 2009 5:05 AM > > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in > Liter Scale > > > > Dear Dr Ku, > > > > It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg > and 1mM EDTA. > > > > And I want to try other affinity columns, just don't know what resin would > be appropriate. > > > > Sincerely, > > > > Xuan Yang > > > > 2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de> > > Can you put a 6His-tag on your protein and add some Ni-NTA beads to your > solution to capture (and concentrate) the properly folded molecules? > > Quoting "Xuan Yang" <pattisy...@gmail.com>: > > Dear All, > > I am working on protein refolding via dialysis in large volumn > (typically 2~4 litters). It was problematic when I wanted to concentrate the > solution to at least less than 500ml. If you know any device appropriate for > such task, please help me out:) > > Thanks in advance! > > Sincerely, > > Xuan Yang > > > > -- Pius S Padayatti Scientist, Polgenix, Inc. 11000 Cedar Ave, Suite 260 Cleveland, OH 44106 Phone: 216-658-4528 Fax: 216-658-4529
