If you want to just reduce the volume/concentrate proteins, you can use Millipore's Stirred cell (high-output stirred cell, 2,000 ml capacity).
Zhongren Wu ________________________________ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Xuan Yang Sent: Thursday, September 03, 2009 5:05 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale Dear Dr Ku, It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg and 1mM EDTA. And I want to try other affinity columns, just don't know what resin would be appropriate. Sincerely, Xuan Yang 2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de> Can you put a 6His-tag on your protein and add some Ni-NTA beads to your solution to capture (and concentrate) the properly folded molecules? Quoting "Xuan Yang" <pattisy...@gmail.com>: Dear All, I am working on protein refolding via dialysis in large volumn (typically 2~4 litters). It was problematic when I wanted to concentrate the solution to at least less than 500ml. If you know any device appropriate for such task, please help me out:) Thanks in advance! Sincerely, Xuan Yang
