Hi,
Could you clarify your request a bit? Generally speaking if you allow the ectodomain to have a membrane anchoring segment it will 'compromise' solubility since the resulting protein most likely requires special treatment (detergents) in order to be soluble. There's also the matter of the secretion signal - depending on your expression system you may succeed using the original secretion signal, or you may have to engineer an entirely new one. Artem "Nothing is built on stone; all is built on sand, but we must build as if the sand were stone" Jorge Luis Borges _____ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of aka akaka Sent: Sunday, September 06, 2009 8:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Adding a transmembrane segment Dear All I am working with a viral protein that contains an ectodomain, a TM segment and a cytoplasmic region. I was wondering if there is a general strategy that allows the addition of the transmembrane segment to the ectodomain without compromising solubility and secretion. Thanks Dr. R. Depetris Weill Medical College of Cornell University _____
