Hello Jerry,
We use the Van Duyne et al feedback inhibition method with BL21
and have never had a problem.
ho
UC Berkeley
--
Date:Thu, 26 Mar 2009 14:58:57 -0700
From:Jerry McCully
Subject: selenomethionine labeling
--_6abd9eb3-ba7b-436a-99d3-053f7f3a6
I am not sure this is the answer for your question, but please check,
http://www.nusrc.nagoya-u.ac.jp/WatanabeLab/XtalMount/index.html
We named it as a "loopless" mounting method in the paper, but now we
leave the loop...
The vacuum cleaner I have used for the method is "my mouth". But we
h
If I understand you correctly, you are writing about sucking a crystal up
into a small capillary. This is an ancient technique used with the very
first protein crystal diffraction data collection in the 1950s and 1960s
that is still used today. You can use a small 1 ml plastic syringe for the
pos
Try a non-auxotrophic method, in BL21(DE3):
http://www.xtals.org/pdfs/selenium.pdf
Artem
---
When the Weasel comes to give New Year's greetings to the Chickens no good
intentions are in his mind.
_
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jerry
Dear All,
Sorry to disturb you with this simple question. I am not familiar with the
membrane protein.
Here I just ask that is Integrin alpha9beta1 or other integrin protein
soluble in water or Tris-HCl buffer? If I want to put integrin alpha9beta1
and its ligand in Tris-HCl buffer(PH7.4) to stud
Dear All:
I got a problem with the expression of one N-terminal His6 tagged protein
in B834 cells when I tried to do selenomethionine labeling.
However, the protein can be expressed in BL21{DE3) cells.
Welcome any troubleshooting tips.
All the best,
Jerry
__
Hi Klaas,
a very concise description of your problem!
Actually, you don't need to install arpwarp through the ccp4i interface.
Once you setup your ccp4 environment, you can just run the install.sh (or
install.csh in your case) which is in the arp-warp directory and
everything should be set up
I was testing FPARTi,PHIPi in refmac 5.5.0089 and got an unexpected
result. With scaling off (no SCPART keywords), adding an FPART1,PHIP1
where all entries for both columns were 0 gives Rwork and Rfree of 1.0.
When scaling of FPART is turned on, the Rwork,Rfree values are as
expected (comparible
Problems installing tasks to CCP4i.Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized capillaries,
such as are pulled by patch-pipet machines, or those used in microfluidics? The
material could be either glass or plastic, and one could have some method of
Dear colleagues,
I have problems installing external tasks to CCP4i. This particularly
involves ARP/wARP 7.0.1. Despite the error message shown by the
install/uninstall form after setting the location of the task archive file,
I assume that the problem is due to an error or incompatibility with my
Hi,
Phosphate + calcium - not an easy combination :)
Fluka brand PEGs used to be the way to go.
I would carefully test the pH of the actual solution in the kit, and
compare it with the pH of the solutions you're making yourself. There are
several reasons as to why - but these values aren't alway
Hello,
I am trying to reproduce two hits obtained from the PEG/Ion screen of
Hampton which showed up with 20% PEG3350 and 0.2M K2HPO4/0.2M CaCl2. I
tried optimizations making up my own solutions with the two salts from Sigma
(The PEG is from Hampton). To my surprise, the hits do not translate
We learnt from Flip Hoedemakers that wine is supposed to be kept at
constant temperature without vibrations and that wine coolers make very
fine crystal incubators.
Few years ago we bought three wine coolers at a local shop and they work
well (for growing crystals that is). It is worthwhile to chec
Hello,
I have a slightly different suggestion which we have found works best
and cheap. We have taken old (non-working) refrigerators and place them
inside the cold rooms *"but never power them"*. Since they are
refrigerators they have a good insulation to start with , have trays to
place crys
A permament crystallography team leader position is being advertised at the
AstraZeneca research site in Boston, USA. The job involves structure-based
drug design support for local infection and oncology activities, and
supervising a new team of two highly talented crystallographers. The team
But aren't the bump restraints re-evaluated at each iteration? That was
my understanding (but I could be wrong), after all it's not a big deal
to compute interatomic distances. One contribution that is not
re-evaluated every iteration but only at iteration 1 is (I believe, but
again I could be wr
Kristof Van Hecke wrote:
I want to optimize a DNA-helix with the function "Structure
idealisation" in Refmac_5.5.00782 (CCP4_6.1.1).
My question, is this performing just a geometry optimization (against
a library), or is there also an energy-optimization of some kind
involved,..?
What's the di
17 matches
Mail list logo
