Try a non-auxotrophic method, in BL21(DE3):
http://www.xtals.org/pdfs/selenium.pdf Artem --- When the Weasel comes to give New Year's greetings to the Chickens no good intentions are in his mind. _____ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jerry McCully Sent: Thursday, March 26, 2009 5:59 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] selenomethionine labeling Dear All: I got a problem with the expression of one N-terminal His6 tagged protein in B834 cells when I tried to do selenomethionine labeling. However, the protein can be expressed in BL21{DE3) cells. Welcome any troubleshooting tips. All the best, Jerry _____ Quick access to Windows Live and your favorite MSN content with Internet Explorer 8. <http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MS N55C0701A>
