Hello, I am trying to reproduce two hits obtained from the PEG/Ion screen of Hampton which showed up with 20% PEG3350 and 0.2M K2HPO4/0.2M CaCl2. I tried optimizations making up my own solutions with the two salts from Sigma (The PEG is from Hampton). To my surprise, the hits do not translate during optimizations. I am suspecting the salts. My question is, what grade of Sigma chmicals would be equivalent to the ones sold by Hampton?
Any other suggestions would be more than welcome. Regards Hari
