Hi Bruce,

As requested, I'm transferring over the entire folder. It's saved as
140107OTIS_from_Shepherd_attn_Fischl.tar.gz

Our pipeline appears to sidestep aseg (I'm not precisely sure why or how).
I have what I think are a reasonable brainmask.mgz and wm.mgz, which appear
to be the two key files to target for manual edits. But when I proceed to
the next step:
   recon-all -autorecon2-wm -subjid 140107OTIS -cc-crs 128 108 168
-pons-crs 128 255 128 -noaseg
there are divots in the grey matter in the ACC just anterior and dorsal to
the genu and in middle OFC. I'm not sure why. Additionally, the wm edits in
occipital do not appear to be helping. This is easiest to see if I run
   tkmedit 140107OTIS brain.mgz -aux wm.mgz -surfs
select a point just to the side of midline (e.g. 125 103 128) and switch to
a sagittal view.

Thanks for your help.

stephen



*stephen v. shepherd   phd*
The Rockefeller University / 1230 York Avenue / New York NY 10065-6307 USA
// 212.327.7620


On Wed, Apr 16, 2014 at 10:05 AM, Bruce Fischl
<fis...@nmr.mgh.harvard.edu>wrote:

> Hi Stephen
>
> can you upload the whole subject dir? Also, why is there no aseg for this
> subject?
>
> Bruce
> On Tue, 8 Apr 2014, S.V.Shepherd [work] wrote:
>
>  I believe ventricles and callosum are correctly labeled. I've been
>> viewing with ''' tkmedit 140107OTIS brain.mgz -aux wm.mgz
>> -surfs '''.  I've uploaded ( as per the instructions here:
>> https://surfer.nmr.mgh.harvard.edu/fswiki/FtpFileExchange ) the file
>> contents of my Otis's mri/ directory, as 
>> SVSHEPHERD_subj_OTIS_for_DOUG_on_20140408.tar.gz.
>> I hope this will be enough?
>>
>> Ares of concern are
>> * ACC (e.g. 123 105 208) - maybe misses a thin wm bundle? or won't grow
>> grey from CC?
>> * OFC (e.g. 123 133 218) - may be excluded as Olfactory Bulb, or bc too
>> bright.
>> * Occ (e.g. 115 109 97) - mostly poor scan quality; may require manual
>> overpaint.
>>
>> stephen
>>
>>       Hi Stephen
>>
>>       Are the ventricles and callosum correctly labeled? If you upload a
>> subject I'll
>>       try to take a look
>>       Cheers
>>       Bruce
>>
>>
>>       > On Apr 7, 2014, at 8:38 PM, "S.V.Shepherd [work]"
>>       > <stephen.v.sheph...@gmail.com> wrote:
>>       >
>>       > Greetings,
>>       >
>>       > I've been reconstructing a surface in an NHP using a Kolster T1
>> scan and
>>       > FS5.3.  It went very well, at first, but I've hit a wall.  I have
>> trouble in
>>       > occipital (due to poor signal strength/constrast) and some
>> typical-with-NHP
>>       > problems in ACC and OFC.  I think there are three related
>> questions.
>>       >
>>       > 1) I lose parts of the ACC near the genu of the corpus callosum.
>> I have
>>       > attached a screen shot -- the white matter and gray matter both
>> look decent.
>>       > I wondered if the corpus callosum might be handled specially, and
>> if
>>       > differences between human and nhp callosi might prevent the ACC
>> from growing
>>       > normally?
>>       >
>>       > 2) In trying to deal with this, I greyed out the ACA, which was
>> bright and
>>       > potentially confounding. I wasn't sure how to do this in
>>       > brain.finalsurfs.manedit, since my understanding is that only 1s
>> (nonbrain)
>>       > and 255s (wm) were preserved. I tried brain, which got
>> overwritten, but
>>       > ultimately succeeded by editing brainmask and rerunning
>> -autorecon2-wm.  Is
>>       > there a way to repain gray matter in brain.finalsurfs.manedit?
>> Alternately,
>>       > if I continue editing brainmask (or equivalent) directly, should
>> I be
>>       > darkening or lightening the gray matter in the excluded section
>> of the ACC?
>>       > I would assume lighten -- but in the OFC, some gray matter (not
>> shown)
>>       > appears to be excluded for being too bright.
>>       >
>>       > 3) In some places I've specified wm (e.g. in the ACC, here) but
>> it hasn't
>>       > stuck. I assume this is because autorecon isn't convinced by the
>> wm
>>       > specification after looking at brain.finalsurfs -- how might I
>> (gently!)
>>       > override this?
>>       >
>>       > Thanks very much for your time and patience.
>>       >
>>       > stephen
>>       >
>>       > <Capture1.PNG><Capture2.PNG>
>>
>>
>>
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