These are my go-to review articles for ideas for getting a ligand into a crystal.
https://scripts.iucr.org/cgi-bin/paper?S0907444906047020 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297911/ I have also seen a talk where someone successfully dissolved their water-insoluble ligand in Octan-1-ol, and layered that solution over the drop after crystals had grown. They incubated for X period of time (I don't recall) froze the crystals and found the ligand had diffused into the crystals and was in the active site. Maybe someone else knows of the/a paper describing this? Hope this helps, D -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == Diamond User Committee MX representative == about.me/david_briggs ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Gourab Basu Choudhury <goura...@csiriicb.res.in> Sent: 24 April 2021 03:46 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Co crystalization with less soluble ligand. External Sender: Use caution. Hello everyone, I am finding it difficult for getting a ligand density inside the protein as the ligand is very much insoluble. It's only soluble in 100% DMSO. I tried for co crystalization. Kd value of the ligand is near 40um. Any suggestion what to do? ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=04%7C01%7C%7Ce31fd41ae0d94d54f04108d906cc8c65%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637548297877580067%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=dSgnMbz9Zrqhw3oPKKs51HY2Zbr7ZAr4rmk1KIqFCJE%3D&reserved=0> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
