And then of course, you need to decide whether you at all care to know anything about a compound that is so insoluble that it needs that kind of treatment .... :)

On 23/06/2021 09:52, hoh wrote:
Hi

As total insolubility does not exist, I regularly use another method, which is to deposit a grain of the ligand directly into the drop.

In this case, we no longer control the concentration. The goal is to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the results, it is possible to adjust the time. If after one hour, the crystal no longer diffracts, redo the experience by freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1 week. This technique needs 2 things
, time and several exploitable crystals in the drop.


FH


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