Hi Frank, heme becomes near completely insoluble when it dimerizes. Fatty acids are also very insoluble. It is quite hard to get them into HSA, as far as I remember.
Cheers, Tim On Wed, 23 Jun 2021 10:33:42 +0100 Frank von Delft <frank.vonde...@cmd.ox.ac.uk> wrote: > And then of course, you need to decide whether you at all care to > know anything about a compound that is so insoluble that it needs > that kind of treatment .... :) > > On 23/06/2021 09:52, hoh wrote: > > Hi > > > > As total insolubility does not exist, I regularly use another > > method, which is to deposit a grain of the ligand directly into the > > drop. > > > > In this case, we no longer control the concentration. The goal is > > to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending > > on the results, it is possible to adjust the time. If after one > > hour, the crystal no longer diffracts, redo the experience by > > freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1 > > week. This technique needs 2 things > > , time and several exploitable crystals in the drop. > > > > > > FH > > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ -- -- Tim Gruene Head of the Centre for X-ray Structure Analysis Faculty of Chemistry University of Vienna Phone: +43-1-4277-70202 GPG Key ID = A46BEE1A ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
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