Hello Gourab, DMSO is not the only possible solvent- there are others to try. I don't want to sound negative, but 40 micromolar is not a very tight binding compound. It would also depend on how that binding constant was measured- how did someone get enough in solution to measure that? Best of luck, tom
Tom Peat, PhD Proteins Group Biomedical Program, CSIRO 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 tom.p...@csiro.au ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Gourab Basu Choudhury <goura...@csiriicb.res.in> Sent: Saturday, April 24, 2021 12:46 PM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Co crystalization with less soluble ligand. Hello everyone, I am finding it difficult for getting a ligand density inside the protein as the ligand is very much insoluble. It's only soluble in 100% DMSO. I tried for co crystalization. Kd value of the ligand is near 40um. Any suggestion what to do? ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
