Fatty acids are very soluble in glycerol or the PEGs.
Most proteins tolerate high concentrations (30-50%) glycerol and PEGs.�
So, it is quite possible to make a solution that will allow the protein
crsytal and the ligand to both be in the same solution.
One of my former colleagues was in charge of dosing mice for drug
testing.� He often had to get hydrophobic compounds into solution long
enough to feed the compounds to the mice.
If the key compound is not soluble in the current system, change the
system.
Some might complain that this is a lot of work (which it might be), but
that might also be why no one else has done it yet.
On 6/23/2021 5:53 AM, Tim Gruene wrote:
Hi Frank,
heme becomes near completely insoluble when it dimerizes. Fatty acids
are also very insoluble. It is quite hard to get them into HSA, as far
as I remember.
Cheers,
Tim
On Wed, 23 Jun 2021 10:33:42 +0100 Frank von
Delft <frank.vonde...@cmd.ox.ac.uk> wrote:
And then of course, you need to decide whether you at all care to
know anything about a compound that is so insoluble that it needs
that kind of treatment .... :)
On 23/06/2021 09:52, hoh wrote:
Hi
As total insolubility does not exist, I regularly use another
method, which is to deposit a grain of the ligand directly into the
drop.
In this case, we no longer control the concentration. The goal is
to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending
on the results, it is possible to adjust the time. If after one
hour, the crystal no longer diffracts, redo the experience by
freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1
week. This technique needs 2 things
, time and several exploitable crystals in the drop.
FH
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