Dear Mike
Even Knauer (though known for HPLCs) are up with the Bioline
chromatography solutions.
And cheaper than GE.
With best regards
Kris
On Thu, Oct 13, 2011 at 6:48 AM, Paul Smith wrote:
> Michael,
> Unfortunately, I actually don't know who serves these machines apart from
> GE.
> Because yo
Yet Uaniso's are multiplied by 1 and stored as integers with no problem!
-- Ian
On Thu, Oct 13, 2011 at 1:44 AM, James Holton wrote:
> I think the PDB decided to store "B" instead of "U" because unless the
> B factor was > 80, there would always be a leading "0." in that
> column, and that w
Hi Stephen
You just multiply the Uij values in the ANISOU record by 8pi^2/1.
This begs the question why you want the Bij values in the first place
(since the Bij's don't correspond to any physical parameter)? Note
that TLS parameters are on the same scale as U, not B.
Cheers
-- Ian
2011/1
Hi,
Before coming to my current lab, I had used a Prime for a couple of years. Did
the job, no complaints. Since being exposed to the Akta Express though, I
would have to say that this is a great machine for purification of multiple
constructs at the same(ish) time and I would really, really
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I am glad the structures that have been solved using the
free-lunch-algorithm as implemented in shelxe did not know they were not
allowed to be solved. Of course there is DM involved, as has been
pointed out ;-)
On 10/12/2011 10:12 PM, Edward A. Berry
On 13/10/11 04:25, Dr. STEPHEN SIN-YIN, CHUI wrote:
> Dear All,
>
> For all monomers (3 letter) used in COOT, where can i find the full names of
> the
> whole library? Many thanks
>
In the console, coot tells you which file it has read in, you can read
that file and look for _chem_comp.name
0.7
Dear all,
A Postdoctoral Position is available in the Macromolecular Crystallography
beamline (BL13 XALOC) at the Alba Synchrotron.
About BL13-XALOC beamline and Alba synchrotron
Alba is a 3rd generation, 3-GeV synchrotron facility close to Barcelona
(Catalonia, Spain). It is currentl
Okinawa Institute of Technology/CCP4 School on Protein Crystallography Software
This is just a reminder that the deadline for applications for the OIST/CCP4
school: From data processing to structure refinement and beyond, is October
25th.
A full provisional program and the list of speakers is n
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Dear all,
I would like to draw your (at least the German speaking readers')
attention to the link
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where you can vote for my favourite sports club, Waspo 08 Goettingen.
Ing-DiBa will donate 1000E
hello everyone:
Excuse me, could anyone give me some suggestions? Afetr several cycles
of refmac, it give me such a result:
InitialFinal
R factor0.2540 0.2531
R free0.3499 0.3500
Rms BondLeng
Possibility of twinning? A simple way to check this at this point is to turn
on the twin option in refmac and examine the log file. Twinning can cause
you to get stuck at high R values in unaccounted for.
Roger Rowlett
On Oct 13, 2011 7:14 AM, "王瑞" wrote:
> hello everyone:
>
> Excuse me, cou
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Dear ,
- - how complete is your model compared to the sequence expected in the
crystal? If it is not very complete, yet, your R-values might diverge
- - Your RMS BondAngle is pretty high. Set the matrix weight manually to a
lower number (0.1 or less)
On 10/13/2011 12:14 PM, 王瑞 wrote:
hello everyone:
Excuse me, could anyone give me some suggestions? Afetr several cycles
of refmac, it give me such a result:
InitialFinal
R factor0.2540 0.2531
R f
Hi Francois,
As for point 2 in your list: There are crystallographers (those who deal with
quasi-crystals, such as the recent Nobel laurate Dan Shechtman) who live, so to
speak, outside the box that you're referring to. There is no periodicity in
their quasi-crystals and even the unit cell i
Echoing whats been said:
1- Are you sure your crystal really is in C 2 2 21? If so How good is your data
(completeness, Rmerge, etc...)
2-Could have twinning? I recently just got done working on a structure that
could be scaled in C 2 2 21 but turned out to really be an almost perfect P21
twin.
GE has a policy on Product Obsolescence, which, afaik, means that service
contracts will not be issued to those instruments that were discontinued 7
years ago. Among instruments affected by this deadline are "AktaPrime INCL",
"AktaPrime EXCL" and "AktaPrime COMPLETE". You have time to service th
having experience with both, I find Akta and Biorad both do the job, with the
Akta equipment giving a sturdier impression.
Neither of them is cheap, and given similar price I would go for an Akta when
buying new - however, if a significant price advantage exists, I would have no
problem choosing
Similar to Tim's suggestion, but your low resolution limit may be too
low (check refmac's chart of R vs resolution to confirm this).
Pete
王瑞 wrote:
hello everyone:
Excuse me, could anyone give me some suggestions? Afetr several cycles of
refmac, it give me such a result:
We utilize a local company and bundle almost all service contracts through
them (Spectrofuge - RTP area, NC). Preventative maintenance and repairs are
covered on ATKAs, centrifuges, scintillation counters and so on (pretty much
all major equipment, minus x-rays of course). Being local, they schedul
On Wed, 2011-10-12 at 18:18 -0700, Paul Smith wrote:
> and have heard some good things about Bio-rad
Personally, my experience with Biorad was very positive. For instance,
we had the switch valve motor burn out and they sent us the motor for
$150 or so while they could have charged us full price
On Thu, 2011-10-13 at 11:25 +0800, Dr. STEPHEN SIN-YIN, CHUI wrote:
> Dear All,
>
> For all monomers (3 letter) used in COOT, where can i find the full names of
> the
> whole library? Many thanks
>
> stephen
>
Assuming that you have ccp4 configured, you can use this one-liner
find $CCP4_LI
Dear Yuri,
Could you give out the details? Such as how you found the twins?
2-Could have twinning? I recently just got done working on a structure that
could be scaled in C 2 2 21 but turned out to really be an almost perfect P21
twin.
(of course in monoclinic there are certain conditions for tw
As a personal touch, I also find Biorad software much more intuitive.
This discussion, of course, is similar to comparing car brands -
experiences differ.
What mystifies me is a need for a service maintenance contract at all. Has
hardware become so much less reliable than in the past?
Grante
On Thu, 2011-10-13 at 11:25 +0800, Dr. STEPHEN SIN-YIN, CHUI wrote:
Dear All,
For all monomers (3 letter) used in COOT, where can i find the full names of the
whole library? Many thanks
stephen
Assuming that you have ccp4 configured, you can use this one-liner
find $CCP4_LIB/data/monomers -
I have used and worked on both the Bio-Rad and Akta systems. It is
really a matter of opinion, but they both get the job done.
Exterior:
The Akta systems look nice with metal exteriors while the Bio-Rads look
a little cheap being plastic. Both are really heavy, though.
Interior:
Once you take
After L-test,the result are follows:
TWINNING ANALYSIS:
First principles calculation of potential twinning operators using code by
Andrey Lebedev:
First principles calculation has found 0 potential twinning operators
No twinning detected
$TABLE: L test for twinning:
$GRAPHS: cumulative distribu
Dear users,
Can the optimization of the X-ray weighing factor
and B-factor (overall wt) as mentioned in the paper
Acta Cryst. (2007). D63, 12741281 by Dr.Ian Tickel,
be used for the refinement of the data sets beyond
the resolution range mentioned in the paper: 1.33 -
2.55 Ang?
Also the structur
try a frozen xtal ...
On Fri, 14 Oct 2011 13:12:12 +0800
ChenTiantian wrote:
> Hi there,
> I am processing a dataset which has bad ice rings (as you
> can see in the
> attach png file).
> I tried both XDS and imosflm, and got similar results, it
> seems that adding
> " EXCLUDE_RESOLUTION_RANGE"
First of all, are you sure those are ice rings? They do not look typical. I
think you might have salt crystals from dehydration *before* freezing.
Otherwise, I think your freezing went well. Maybe try a humidity controlled
environment when you freeze.
Second, I'm not so sure the bad stats come
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