Possibility of twinning? A simple way to check this at this point is to turn on the twin option in refmac and examine the log file. Twinning can cause you to get stuck at high R values in unaccounted for.
Roger Rowlett On Oct 13, 2011 7:14 AM, "王瑞" <wangrui...@gmail.com> wrote: > hello everyone: > > Excuse me, could anyone give me some suggestions? Afetr several cycles > of refmac, it give me such a result: > Initial Final > R factor 0.2540 0.2531 > R free 0.3499 0.3500 > Rms BondLength 0.0151 0.0151 > Rms BondAngle 2.0266 2.0393 > Rms ChirVolume 0.1179 0.1188 > In fact, I have tried model building & continue refmac, but the R free is > still above 0.34. The resolution is 43.728 to 2.088Å and space group > is C 2 2 21 . What I should to do? > > Thanks >
