Similar to Tim's suggestion, but your low resolution limit may be too low (check refmac's chart of R vs resolution to confirm this).

Pete

王瑞 wrote:
hello everyone:

    Excuse me, could anyone give me some suggestions? Afetr several cycles of 
refmac, it give me such a result:
                                       Initial    Final
                    R factor    0.2540   0.2531
                       R free    0.3499   0.3500
     Rms BondLength    0.0151   0.0151
      Rms BondAngle    2.0266   2.0393
     Rms ChirVolume    0.1179   0.1188
 In fact, I have tried model building & continue refmac, but the R free is 
still above 0.34. The resolution is  43.728 to  2.088Å and space group is C 2 2 21 
. What I should to do?

Thanks

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