nanodrop system is wonderful. it helps very much in solutions with
high interference from detergents etc etc.
i highly receommend nanodrop specs
PSP
On Thu, Dec 4, 2008 at 10:16 AM, Tim Gruene <[EMAIL PROTECTED]> wrote:
> Dear all,
>
> we would like to purchase a UV spectrometer for measuring pr
We have an Ocean Optics USB-4000 unit in our lab. It does everything
from quantifying protein and nucleic acids, spectrophotometric
titrations, and metalloenzyme spectra at low volume/concentration. It's
not a toy, but a diode array spectrophotometer that has excellent S/N
and resolution. You c
I want to add I absotely hate the nanodrop. We've had a demo for it, and
found the readouts to be very unreliable. Fluctuations of 20% and more.
Just leaving the same drop in and measuring the sample multiple times gives
different values (going in both directions, so not only due to
evaportations
If you reduce the path by a factor of 50, can you not increase the
concentration by the same factor without violating the shadowing
assumption?
Mike
On Thu, Dec 4, 2008 at 10:48 AM, Patrick Loll <[EMAIL PROTECTED]> wrote:
> At the risk of dragging this discussion even further afield from
> cryst
We too have a nano-drop. We really like it , but have not yet fully
switched over.
I agree with all the good things said about it , but here are the few times
the nano drop falls short:
1) We still use the old spec ( 1 cm path length ) for things at a very low
concentration , i.e for the monitor
Wow, that's like putting a pool in your backyard so you don't have to
pay the $3.00 admission every day (I'm kidding). In any event, Ocean
Optics has some very nice, small, and portable units that would run
around $3000 total. These connect to the USB port in a computer and
produce data that
One more issue regarding the Nanodrop: one has to work quite quickly
to avoid potential evaporation. Before buying such an instrument, I
would strongly recommend a demo and careful comparisons between the
Nanodrop and a good, conventional spectrometer with a representative
range of samples.
At the risk of dragging this discussion even further afield from
crystallography:
How can you get realistic numbers for concentrated solutions using
the Nanodrop? I understand that the instrument reduces absorbance by
using a very short path length. However, I thought that in order for
t
We also like the Nanodrop. Very fast, no cuvettes (breaking, washing,
cleaning, uh nitric acid bath anyone?), and the .ndv data file is a
delimited text file. Open in a text editor, copy and paste into a
spreadsheet, and you have a convenient record of all of your stocks,
including date, sample n
CCP4 bulletin board wrote on 12/04/2008 10:16:02
AM:
> Dear all,
>
> we would like to purchase a UV spectrometer for measuring protein
> concentrations (280nm), and I would like to here your comments and
> especially recommendations.
>
> We don't need anything fancy, a small, fast device would be
We use a Beckman Coulter DU730. It has a small footprint if lab space is an
issue. It will do single-wavelength, multi-wavelength, or take an entire
spectrum in 0.5 nm steps if you desire. It comes with the standard 1 ml
cuvette holder, but we also purchased the microcuvette accessory for volume
I would also recommend the nanodrop. It takes a whole spectra every
measurement and there is no need to dilute your sample. You can demo it for
a week and try it out.
James M. Vergis, Ph.D.
University of Virginia Molecular Physiology and Bi
Tim - I would recommend a spectrometer that records entire spectra,
instead of one that takes readings at just 280 nm. Contributions from
light scattering can be very strong and can give results that deviate
from the true value by a factor of two or more. One cannot detect
scattering withou
Dear Tim --
On 4 Dec 2008, at 15:16, Tim Gruene wrote:
we would like to purchase a UV spectrometer for measuring protein
concentrations (280nm), and I would like to here your comments and
especially recommendations.
I love the Nanodrop system... I know you said you don't want anything
fan
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