One more issue regarding the Nanodrop: one has to work quite quickly
to avoid potential evaporation. Before buying such an instrument, I
would strongly recommend a demo and careful comparisons between the
Nanodrop and a good, conventional spectrometer with a representative
range of samples. Convenience is one thing...
Best - MM
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Mischa Machius, PhD
Associate Professor
Department of Biochemistry
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.; ND10.214A
Dallas, TX 75390-8816; U.S.A.
Tel: +1 214 645 6381
Fax: +1 214 645 6353
On Dec 4, 2008, at 12:48 PM, Patrick Loll wrote:
At the risk of dragging this discussion even further afield from
crystallography:
How can you get realistic numbers for concentrated solutions using
the Nanodrop? I understand that the instrument reduces absorbance
by using a very short path length. However, I thought that in order
for the Beer-Lambert formalism to be applicable, the solution needs
to be sufficiently dilute so that the chance of molecules
"shadowing" one another is negligible. Isn't this condition violated
for concentrated solutions (even with short path lengths)?
Pat
On 4 Dec 2008, at 1:27 PM, Michael Giffin wrote:
We also like the Nanodrop...
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Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
(215) 762-7706
[EMAIL PROTECTED]
