If you reduce the path by a factor of 50, can you not increase the concentration by the same factor without violating the shadowing assumption?
Mike On Thu, Dec 4, 2008 at 10:48 AM, Patrick Loll <[EMAIL PROTECTED]> wrote: > At the risk of dragging this discussion even further afield from > crystallography: > How can you get realistic numbers for concentrated solutions using the > Nanodrop? I understand that the instrument reduces absorbance by using a > very short path length. However, I thought that in order for the > Beer-Lambert formalism to be applicable, the solution needs to be > sufficiently dilute so that the chance of molecules "shadowing" one another > is negligible. Isn't this condition violated for concentrated solutions > (even with short path lengths)? > Pat > On 4 Dec 2008, at 1:27 PM, Michael Giffin wrote: > > We also like the Nanodrop... > > --------------------------------------------------------------------------------------- > > Patrick J. Loll, Ph. D. > > Professor of Biochemistry & Molecular Biology > > Director, Biochemistry Graduate Program > > Drexel University College of Medicine > > Room 10-102 New College Building > > 245 N. 15th St., Mailstop 497 > > Philadelphia, PA 19102-1192 USA > > (215) 762-7706 > > [EMAIL PROTECTED] >
