We too have a nano-drop. We really like it , but have not yet fully switched over.
I agree with all the good things said about it , but here are the few times the nano drop falls short: 1) We still use the old spec ( 1 cm path length ) for things at a very low concentration , i.e for the monitoring free thiols in protein with ellman reagent , the absorbances are very low and give poor reproducibility on the nanodrop because of small path-length . Of course this can be overcome by using a lot of protein at a higher concentration and modifying the assay. 2) For very concentrated membrane protein samples which tend to have a large concentration of detergent . The reproducibility is not very good because of the high concentration of deteregent preventing a proper meniscus from forming. The solution to this is to dilute your sample so the dteergent concentration is manageable ( a few times the CMC instead of tens of times the CMC Other than for these issues we almost entirely use the nanodrop and would gladly recommend it Hari On Thu, Dec 4, 2008 at 1:27 PM, Michael Giffin <[EMAIL PROTECTED]> wrote: > We also like the Nanodrop. Very fast, no cuvettes (breaking, washing, > cleaning, uh nitric acid bath anyone?), and the .ndv data file is a > delimited text file. Open in a text editor, copy and paste into a > spreadsheet, and you have a convenient record of all of your stocks, > including date, sample name, concentration, and full spectra. > > It is expensive, but so are good cuvettes. > > > Mike > > > Michael Giffin > The Scripps Research Institute > Department of Molecular and Experimental Medicine > 10550 North Torrey Pines Road, MEM-131 > La Jolla, CA 92037 > email: [EMAIL PROTECTED] > lab: 858-784-7758 > > On Thu, Dec 4, 2008 at 7:16 AM, Tim Gruene <[EMAIL PROTECTED]> > wrote: > > Dear all, > > > > we would like to purchase a UV spectrometer for measuring protein > > concentrations (280nm), and I would like to here your comments and > > especially recommendations. > > > > We don't need anything fancy, a small, fast device would be sufficient. > > > > Tim > > > > > > -- > > Tim Gruene > > Institut fuer anorganische Chemie > > Tammannstr. 4 > > D-37077 Goettingen > > > > GPG Key ID = A46BEE1A > > >
