on behalf of Saniya Dubey
<f97235562ca7-dmarc-requ...@jiscmail.ac.uk>
Date: Monday, December 23, 2024 at 4:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Recommendations for crystallization screens
Hello Everyone,
Thank you for all your responses. We would still like to hea
Hello Everyone,
Thank you for all your responses. We would still like to hear from
others who might have additional recommendations and experiences to
share with us.
Thank you once again for the valuable inputs.
Regards,
Saniya Dubey
PhD Student
Macromolecular Structural Biology Lab,
Indian Inst
Hi Saniya,
Another place to start is molecular dimensions - they say for
'your initial crystallisation screen, please use our Screen Selection
Guide and Screen Redundancy Guide to maximise the crystallisation
chemical space sampled and increase your crystallisation success rate'
- I have not tri
Hi Saniya,
Open up the catalog/website of Hampton Research and you will find screens
recommended for these different kinds of samples of protein-ligand
complexes, where ligand=protein, nucleic acid, etc. Depending on the
ligand, screens include components of buffers/salts/precipitants that are
les
Dear Saniya,
For complexes with antibodies (special case of protein-protein
complexes), you may consider the ProPlex screen and the TCR/pMHC
Optimized Protein crystallization screen.
In general, take also an (at least predicted) pI of your protein(s) into
account when selecting a screen. Your p
Hello Saniya,
For protein-DNA complexes, the set of conditions described in this article has
been helpful in the past: https://doi.org/10.1107/S1744309112025316
You will find more info in a message I posted in a discussion from earlier this
year: https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A
Hi Saniya,
In general I don't have a preferred kit to try. I try all the ones that are
available. More you try, more chances you have.
Best wishes
__
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre
