For protein-DNA complexes, we generally found that the ends of the DNA duplex are the most important variable because DNA bases love to stack and usually form crystal-packing contacts. Instead of more screening kits (say, one fairly low salt PEG-ion screen kit should do) buy lots of oligos that you can mix-n-match to get blunt, 1nt and 2nt overhangs (which often base pair with one another in the crystal). Hold your nose and throw in cheap desalted-only oligos, and if you see microcrystals, then see if fancy expensive purification improves them.
Phoebe From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Saniya Dubey <0000f97235562ca7-dmarc-requ...@jiscmail.ac.uk> Date: Monday, December 23, 2024 at 4:41 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Recommendations for crystallization screens Hello Everyone, Thank you for all your responses. We would still like to hear from others who might have additional recommendations and experiences to share with us. Thank you once again for the valuable inputs. Regards, Saniya Dubey PhD Student Macromolecular Structural Biology Lab, Indian Institute of Technology, Hyderabad On Sun, Dec 15, 2024 at 4:35 PM Saniya Dubey <bo20resch11...@iith.ac.in> wrote: > > Dear All, > > We're exploring suitable crystallization screens for different types > of complexes, including: > > 1) Protein-nucleic acid complexes > 2) Protein-protein complexes > 3) Protein-ligand complexes > > We would appreciate any recommendations you might have of specific > screens that work well for these types of samples. > > Thank You for your insights! > > Regards, > Saniya Dubey > PhD Student > Macromolecular Structural Biology Lab, > Indian Institute of Technology, Hyderabad -- Disclaimer:- This footer text is to convey that this email is sent by one of the users of IITH. So, do not mark it as SPAM. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
