Hi Saniya, Open up the catalog/website of Hampton Research and you will find screens recommended for these different kinds of samples of protein-ligand complexes, where ligand=protein, nucleic acid, etc. Depending on the ligand, screens include components of buffers/salts/precipitants that are less likely to destabilize the complex and aid in crystallization (for example, high salts can destabilize protein-DNA complexes). Then you can also cross check with other crystallization screens/companies to get a sense of other options. Many of these screens are built from published papers on suitability of components for specific types of complexes.
Generally speaking, while it could be useful to screen with whatever you have easily available, and to be very thorough screen with all screens you can get your hands on, in reality, this could be limited by available of samples/complexes, and also the effort of screening, imaging, monitoring, following up on hits for optimization, etc. Best regards, Debanu -- Debanu Das, Ph.D. bio.site/debanu_das On Sun, Dec 15, 2024 at 3:16 AM Saniya Dubey < 0000f97235562ca7-dmarc-requ...@jiscmail.ac.uk> wrote: > Dear All, > > We're exploring suitable crystallization screens for different types > of complexes, including: > > 1) Protein-nucleic acid complexes > 2) Protein-protein complexes > 3) Protein-ligand complexes > > We would appreciate any recommendations you might have of specific > screens that work well for these types of samples. > > Thank You for your insights! > > Regards, > Saniya Dubey > PhD Student > Macromolecular Structural Biology Lab, > Indian Institute of Technology, Hyderabad > > -- > > > Disclaimer:- This footer text is to convey that this email is sent by one > of the users of IITH. So, do not mark it as SPAM. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
