Hi Saniya, Another place to start is molecular dimensions - they say for 'your initial crystallisation screen, please use our Screen Selection Guide and Screen Redundancy Guide to maximise the crystallisation chemical space sampled and increase your crystallisation success rate' - I have not tried these yet. Minimizing the amount of floppy DNA not bound by the protein is often important in getting high resolution crystal structures. When I worked for GSK we eventually gel purified and crystallised a DNA complex with a new class of antibiotic [described in Bax, B. D., Chan, P. F., Eggleston, D. S., Fosberry, A., Gentry, D. R., Gorrec, F., ... & Gwynn, M. N. (2010). Type IIA topoisomerase inhibition by a new class of antibacterial agents. Nature, 466(7309), 935-940.]. The success of this work very much depended on excellent work by Claus Spitzfaden who developed a gel filtration assay that ran on 6uls of protein (automatically injected from a 96 well plate). This assay was used to optimize the length of the DNA, the DNA sequence, and the metal ions used - it is described in the supplementary material to the Nature article. Best regards, Ben Bax
On Tue, Dec 17, 2024 at 5:30 PM Debanu <ddas.cons...@gmail.com> wrote: > > Hi Saniya, > > Open up the catalog/website of Hampton Research and you will find screens > recommended for these different kinds of samples of protein-ligand complexes, > where ligand=protein, nucleic acid, etc. Depending on the ligand, screens > include components of buffers/salts/precipitants that are less likely to > destabilize the complex and aid in crystallization (for example, high salts > can destabilize protein-DNA complexes). Then you can also cross check with > other crystallization screens/companies to get a sense of other options. Many > of these screens are built from published papers on suitability of components > for specific types of complexes. > > Generally speaking, while it could be useful to screen with whatever you have > easily available, and to be very thorough screen with all screens you can get > your hands on, in reality, this could be limited by available of > samples/complexes, and also the effort of screening, imaging, monitoring, > following up on hits for optimization, etc. > > Best regards, > Debanu > -- > Debanu Das, Ph.D. > bio.site/debanu_das > > > > On Sun, Dec 15, 2024 at 3:16 AM Saniya Dubey > <0000f97235562ca7-dmarc-requ...@jiscmail.ac.uk> wrote: >> >> Dear All, >> >> We're exploring suitable crystallization screens for different types >> of complexes, including: >> >> 1) Protein-nucleic acid complexes >> 2) Protein-protein complexes >> 3) Protein-ligand complexes >> >> We would appreciate any recommendations you might have of specific >> screens that work well for these types of samples. >> >> Thank You for your insights! >> >> Regards, >> Saniya Dubey >> PhD Student >> Macromolecular Structural Biology Lab, >> Indian Institute of Technology, Hyderabad >> >> -- >> >> >> Disclaimer:- This footer text is to convey that this email is sent by one >> of the users of IITH. So, do not mark it as SPAM. >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing >> list hosted by www.jiscmail.ac.uk, terms & conditions are available at >> https://www.jiscmail.ac.uk/policyandsecurity/ > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
