Hi Saniya,
   Another place to start is molecular dimensions - they say for
'your initial crystallisation screen, please use our Screen Selection
Guide and Screen Redundancy Guide to maximise the crystallisation
chemical space sampled and increase your crystallisation success rate'
- I have not tried these yet.
Minimizing the amount of floppy DNA not bound by the protein is often
important in getting high resolution crystal structures. When I worked
for GSK we eventually gel purified and crystallised a DNA complex with
a new class of antibiotic [described in Bax, B. D., Chan, P. F.,
Eggleston, D. S., Fosberry, A., Gentry, D. R., Gorrec, F., ... &
Gwynn, M. N. (2010). Type IIA topoisomerase inhibition by a new class
of antibacterial agents. Nature, 466(7309), 935-940.]. The success of
this work very much depended on excellent work by Claus Spitzfaden who
developed a gel filtration assay that ran on 6uls of protein
(automatically injected from a 96 well plate). This assay was used to
optimize the length of the DNA, the DNA sequence, and the metal ions
used - it is described in the supplementary material to the Nature
article.
Best regards, Ben Bax

On Tue, Dec 17, 2024 at 5:30 PM Debanu <ddas.cons...@gmail.com> wrote:
>
> Hi Saniya,
>
> Open up the catalog/website of Hampton Research and you will find screens 
> recommended for these different kinds of samples of protein-ligand complexes, 
> where ligand=protein, nucleic acid, etc. Depending on the ligand, screens 
> include components of buffers/salts/precipitants that are less likely to 
> destabilize the complex and aid in crystallization (for example, high salts 
> can destabilize protein-DNA complexes). Then you can also cross check with 
> other crystallization screens/companies to get a sense of other options. Many 
> of these screens are built from published papers on suitability of components 
> for specific types of complexes.
>
> Generally speaking, while it could be useful to screen with whatever you have 
> easily available, and to be very thorough screen with all screens you can get 
> your hands on, in reality, this could be limited by available of 
> samples/complexes, and also the effort of screening, imaging, monitoring, 
> following up on hits for optimization, etc.
>
> Best regards,
> Debanu
> --
> Debanu Das, Ph.D.
> bio.site/debanu_das
>
>
>
> On Sun, Dec 15, 2024 at 3:16 AM Saniya Dubey 
> <0000f97235562ca7-dmarc-requ...@jiscmail.ac.uk> wrote:
>>
>> Dear All,
>>
>> We're exploring suitable crystallization screens for different types
>> of complexes, including:
>>
>> 1) Protein-nucleic acid complexes
>> 2) Protein-protein complexes
>> 3) Protein-ligand complexes
>>
>> We would appreciate any recommendations you might have of specific
>> screens that work well for these types of samples.
>>
>> Thank You for your insights!
>>
>> Regards,
>> Saniya Dubey
>> PhD Student
>> Macromolecular Structural Biology Lab,
>> Indian Institute of Technology, Hyderabad
>>
>> --
>>
>>
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