Dear Yuvaraj,
Jim Pflugrath worked with a few brilliant high school student interns one of
the summers before he retired and made this video to demonstrate how easy it is
to do a ‘halide swish’ to incorporate iodide into crystals of macromolecules
for home laboratory de-novo SAD phasing using C
ed after phaser-EP density modification? Thanks a
lot!!!
Best Regard
Shijun
-原始邮件-
发件人: "Kay Diederichs"
发送时间: 2016年12月28日 星期三
收件人: CCP4BB@JISCMAIL.AC.UK
抄送:
主题: Re: [ccp4bb] SAD phasing
Dear Shijun,
hkl2map is a very nice graphical user interface that makes it easy to use the
件-
>> 发件人: "Kay Diederichs"
>> 发送时间: 2016年12月28日 星期三
>> 收件人: CCP4BB@JISCMAIL.AC.UK
>> 抄送:
>> 主题: Re: [ccp4bb] SAD phasing
>>
>> Dear Shijun,
>>
>> hkl2map is a very nice graphical user interface that makes it easy to use
>>
--
> 发件人: "Kay Diederichs"
> 发送时间: 2016年12月28日 星期三
> 收件人: CCP4BB@JISCMAIL.AC.UK
> 抄送:
> 主题: Re: [ccp4bb] SAD phasing
>
> Dear Shijun,
>
> hkl2map is a very nice graphical user interface that makes it easy to use the
> SHELX programs; I've used it
Dear Shijun,
hkl2map is a very nice graphical user interface that makes it easy to use the
SHELX programs; I've used it successfully around 3A. You find documentation and
download information for SHELX C/D/E and hklmap in the CCP4 community wiki, at
http://strucbio.biologie.uni-konstanz.de/ccp4
Hello everyone
I am learning phasing SAD data now ,and I got some files(like i.phs , .pdb
,fa.res, fa.pdb) when I using SHELXC/D/E,and I know fa.pdb and fa.res (which
contain heavy atom information) are used for the further solution searching
,and .phs file can read by coot. my question
Dear colleagues,
I would like to draw your attention to an upcoming free, educational webinar to
be presented by Tom Edwards, Ph.D. titled "SAD phasing at rotating anode
wavelengths using iodide ions." In this webinar, Tom will present a general
overview of using of iodide ion soaks and (SAD) e
Yep, solved a structure at Cu K-alpha once with the sulfur signal,
resolution 1.7A and a redundancy of 50 or so did the trick. Actually,
one of the sulphurs we found was an ordered SO4 molecule, so if you have
that in your crystallisation condition it might be a bonus...
Flip
Watanabe Nobuhis
If your crystal diffracts well and tough enough for high redundant
data collection, no HA or using sulfur in your protein will be the
first choice. Even at Cu K-alpha. I think...
N. Watanabe
On 2009/10/27, at 3:32, james09 pruza wrote:
Dear All,
I need some suggestions regarding the SAD p
Almost all heavy atoms give a pretty good anom signal at CuKa. Hg Pt Sm
arew great, but Cd and I are useable..
The trick is getting them into the crystal!
Eleanor
Georg Zocher wrote:
Dear James,
A little bit more "old school" but you might also try Uranium,
Samarium and Gadolinium.
In prin
ubject: Re: [ccp4bb] SAD phasing at home source
Dear James,
A little bit more "old school" but you might also try Uranium,
Samarium and Gadolinium.
In principle, take a look what compounds you have in your HA box
and afterwards check anomalous signal for example at
http://skuld.bmsc.wa
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SAD phasing at home source
Dear James,
A little bit more "old school" but you might also try Uranium, Samarium
and Gadolinium.
In principle, take a look what compounds you have in your HA box and
afterwards check anomalous signal for exampl
Dear James,
A little bit more "old school" but you might also try Uranium, Samarium
and Gadolinium.
In principle, take a look what compounds you have in your HA box and
afterwards check anomalous signal for example at
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Best regards,
Georg
, 2009 10:06:35 PM
Subject: Re: [ccp4bb] SAD phasing at home source
Or you can try also substituting anomalous cations into the mix, such as CsI,
take multiple data sets, and do MIRAS:
NaCl
NaI
CsCl
CsI
One would then be able to subtract the various differences to find the HA's. Cs
n.edu
***
- Original Message -
From: James Murphy
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, October 26, 2009 2:15 PM
Subject: Re: [ccp4bb] SAD phasing at home source
I have had success with Iodine, soak your crystal in your cryo + Potassium
I
I have had success with Iodine, soak your crystal in your cryo + Potassium
Iodide
_
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
james09 pruza
Sent: Monday, October 26, 2009 2:33 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] SAD phasing at home source
Dear All,
I need some suggestions regarding the SAD phasing using home source. What
the the most commonly used HA for SAD phasing for Cu anode.
All suggestions are welcome.
Thanks.
James
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