I've had a run of good luck with magic triangle
(5-amino-2,4,6-triiodoisophthalic acid or I3C). Its inexpensive if
you buy the powdered form and make it yourself. And you can
quickly tell if you have the correct structure solution because
the iodines are arranged in equilateral triangles approximately 6
angstroms apart. It also works at room temperature in case you
don't have a cryo system in place. Tobias Beck just recently
published a how-to in Acta (Acta Cryst F65 (10):1068-70).
Good Luck,
Katherine
On Mon Oct 26 16:44:17 EDT 2009, Hong Zhang <zh...@chop.swmed.edu>
wrote:
Xe is also an excellent anomalous scatterer at Cu Ka wavelength.
It is
particularly good for SIRAS phasing since Xe derivative is
usually highly
isomorphous to the native crystals.
Hong
_____________________
Hong Zhang, Ph.D.
Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, Texas 75390-8816
Phone: 214-645-6372
Fax: 214-645-5948
Web: http://hhmi.swmed.edu/Labs/hz/
-----Original Message-----
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On
Behalf Of Georg
Zocher
Sent: Monday, October 26, 2009 3:31 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SAD phasing at home source
Dear James,
A little bit more "old school" but you might also try Uranium,
Samarium and Gadolinium.
In principle, take a look what compounds you have in your HA box
and afterwards check anomalous signal for example at
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Best regards,
Georg
james09 pruza schrieb:
Dear All,
I need some suggestions regarding the SAD phasing using home
source. What the the most commonly used HA for SAD phasing for
Cu anode.
All suggestions are welcome.
Thanks.
James
-- Universit??t T??bingen
Interfakult??res Institut f??r Biochemie
Dr. Georg Zocher
Hoppe-Seyler-Str. 4
72076 Tuebingen
Germany
Fon: +49(0)-7071-2973374
Mail: georg.zoc...@uni-tuebingen.de
http://www.ifib.uni-tuebingen.de
--
SIPPEL,KATHERINE H
Ph. D. candidate
Department of Biochemistry and Molecular Biology
College of Medicine
University of Florida
