Another way can use Iodination of tyrosines. You can use N-Iodo succinamid on existing crystals or on protein prior to crystallization.
Raz ---------------------------------------------------- Raz Zarivach, Ph.D. Department of Life Sciences and the NIBN Ben-Gurion University of the Negev POB 653 Zip code 84105 Beer-Sheva Israel Home page: http://raz189.tripod.com/ tel: +9... fax: +972-8-6472970 skype: zarivach ra...@yahoo.com ---------------------------------------------------- ________________________________ From: Jacob Keller <j-kell...@md.northwestern.edu> To: CCP4BB@JISCMAIL.AC.UK Sent: Mon, October 26, 2009 10:06:35 PM Subject: Re: [ccp4bb] SAD phasing at home source Or you can try also substituting anomalous cations into the mix, such as CsI, take multiple data sets, and do MIRAS: NaCl NaI CsCl CsI One would then be able to subtract the various differences to find the HA's. Cs has a pretty good anomalous signal at CuKa wavelength (~8 electrons). Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu ******************************************* ----- Original Message ----- >From: James Murphy >To: CCP4BB@JISCMAIL.AC.UK >Sent: Monday, October 26, 2009 2:15 PM >Subject: Re: [ccp4bb] SAD phasing at home source > > >I have had success with Iodine, soak your crystal in your cryo + Potassium >Iodide > > > > ________________________________ >From:CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of james09 >pruza >Sent: Monday, October 26, 2009 2:33 PM >To: CCP4BB@JISCMAIL.AC.UK >Subject: [ccp4bb] SAD phasing at home source > >Dear All, > >I need some suggestions regarding the SAD phasing using home source. What the >the most commonly used HA for SAD phasing for Cu anode. >All suggestions are welcome. >Thanks. > >James
