Dear Yuvaraj, Jim Pflugrath worked with a few brilliant high school student interns one of the summers before he retired and made this video to demonstrate how easy it is to do a ‘halide swish’ to incorporate iodide into crystals of macromolecules for home laboratory de-novo SAD phasing using Cu X-ray radiation:
https://www.youtube.com/watch?v=45Qc3jOPaKY Disclaimer: This is not an advert for any product or service by any company, no animals or high school students were harmed in the making of this video. Vegan friendly, Gluten-free, non-GMO. All rights reserved ☺ Good Luck, -Conn Conn Mallett, PhD Senior Account Manager Rigaku Oxford Diffraction Carlsbad, CA 92008 Tel: 7136146891 conn.mall...@rigaku.com<mailto:conn.mall...@rigaku.com> Skype: conn.mallett From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of YUVARAJ I Sent: Tuesday, November 14, 2017 4:59 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Dear all What are the chemicals which give an anomalous signal at home source (Cu Kα radiation) apart from Lathanides and Zinc acetate. What are the things I should taken care of to get sufficient anomalous signal to solve the structure. I have crystal data without anomalous signal at 1.76A resolution. What are theoretical ways possible to solve the structure when there is no homologous structures available in the PDB. Note: There is one cysteine and one methionine in 176 AA length protein. Thanks Yuvaraj --
