The site swift.cmbi.ru.nl/gv/numbers/ is not official, unpublished, and
still poorly maintained. But just for the fun of it, I added a list of
modified cysteines. Be aware that the files are big, so better
download them and read them in the editor than opening them in the browser.
Gert
On 05
CP4 bulletin board [CCP4BB@JISCMAIL.AC.UK
<mailto:CCP4BB@JISCMAIL.AC.UK>] on behalf of Bio Physics
[biophysics.w...@gmail.com
<mailto:biophysics.w...@gmail.com>]
*Sent:* Tuesday, May 12, 2015 11:57 PM
*To:* CCP4BB@JISCMAIL.
84105
>>> Israel
>>> E-mail:
>>> bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype:
>>> boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710*
>>>
>>>
>>>
>
n University of the Negev
> > Beer-Sheva 84105
> > Israel
> >
> > E-mail: bshaa...@bgu.ac.il
> > Phone: 972-8-647-2220 Skype: boaz.shaanan
> > Fax: 972-8-647-2992 or 972-8-646-1710
> >
> >
> >
> >
> > From: CCP4 bulletin board [CCP4
.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Bio
Physics
Gesendet: Mittwoch, 13. Mai 2015 00:16
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] CYS mod
No, it is not sulfenic acid or so, I had that in differend structure. It is L
shaped from S, and linear if
nan Fax: 972-8-647-2992 or 972-8-646-1710*
>>
>>
>>
>>
>>
>> ----------
>> *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio
>> Physics [biophysics.w...@gmail.com]
>> *Sent:* Tue
t;
>
>
>
>
> --
> *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio
> Physics [biophysics.w...@gmail.com]
> *Sent:* Tuesday, May 12, 2015 11:57 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] CYS mod
>
>
t: Tuesday, May 12, 2015 11:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] CYS mod
Hi All,
I have same type of modified CYS !!!
No BME.
On Tue, May 12, 2015 at 2:59 PM, David Schuller
<schul...@cornell.edu> wrote:
So far almost everyone is suggesting BME.
I didn&
So far almost everyone is suggesting BME.
I didn't do the purification & crystallization myself, but I am told:
Buffer was HEPES
cryo was ethylene glycol, glycerol and DMSO.
Some MgSO4 and KPO4
No BME, but maybe some DTT.
Purified from a proteobacterium.
On 05/12/15 15:20, Dyda wrote:
What
BME around ?
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4
Smells like BME to me...
On May 12, 2015 2:32 PM, "David Schuller" wrote:
> What are the most likely modifications of a CYS residue? I am attaching
> images of a couple of residues in my current structure. Blue is 2Fo-Fc,
> Green is Fo-Fc positive difference, purple is model-phased anomalous
> di
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