If I place double bonded O, still there is another blob (1.4A) and density is continuous! Should not be water!!!!!
On Wed, May 13, 2015 at 5:33 AM, Mark J van Raaij <mjvanra...@cnb.csic.es> wrote: > how about SCX with a double-bonded O for the X? That may give the right > angle. > Mark J van Raaij > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > c/Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://www.cnb.csic.es/~mjvanraaij > > > > > > > > > On 13 May 2015, at 00:15, Bio Physics wrote: > > > No, it is not sulfenic acid or so, I had that in differend structure. It > is L shaped from S, and linear if I put SCX mutaion (another 1.4A way a > nice blob). My resolution is 1.3 A. > > > > > > > > On Tue, May 12, 2015 at 4:18 PM, Artem Evdokimov < > artem.evdoki...@gmail.com> wrote: > > Looks like a wrong shape for tetrahedral acid > > ... > > > > On May 12, 2015 4:07 PM, "Boaz Shaanan" <bshaa...@bgu.ac.il> wrote: > > Hi, > > > > > > As I've just written to David, maybe it's sulfenic acid (result of > radiation damage?). I've had those in some cases. See attached scheme for > the chemistry and other possibilities for Cys modification by oxidation. > > > > Boaz > > > > > > > > Boaz Shaanan, Ph.D. > > Dept. of Life Sciences > > Ben-Gurion University of the Negev > > Beer-Sheva 84105 > > Israel > > > > E-mail: bshaa...@bgu.ac.il > > Phone: 972-8-647-2220 Skype: boaz.shaanan > > Fax: 972-8-647-2992 or 972-8-646-1710 > > > > > > > > > > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio > Physics [biophysics.w...@gmail.com] > > Sent: Tuesday, May 12, 2015 11:57 PM > > To: CCP4BB@JISCMAIL.AC.UK > > Subject: Re: [ccp4bb] CYS mod > > > > > > Hi All, > > > > I have same type of modified CYS !!! > > No BME. > > > > On Tue, May 12, 2015 at 2:59 PM, David Schuller <schul...@cornell.edu> > wrote: > > So far almost everyone is suggesting BME. > > > > I didn't do the purification & crystallization myself, but I am told: > > > > Buffer was HEPES > > cryo was ethylene glycol, glycerol and DMSO. > > Some MgSO4 and KPO4 > > No BME, but maybe some DTT. > > > > Purified from a proteobacterium. > > > > > > > > > > On 05/12/15 15:20, Dyda wrote: > > What is in buffer? Perhaps DTT and cacodylate? > > > > Fred > > > [32m******************************************************************************* > > Fred Dyda, Ph.D. Phone:301-402-4496 > > Laboratory of Molecular Biology Fax: 301-496-0201 > > DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov > > Bldg. 5. Room 303 > > Bethesda, MD 20892-0560 URGENT message e-mail: > 2022476...@mms.att.net > > Google maps coords: 39.000597, -77.102102 > > http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred > > > ******************************************************************************* > [m > > > > > > -- > > ======================================================================= > > All Things Serve the Beam > > ======================================================================= > > David J. Schuller > > modern man in a post-modern world > > MacCHESS, Cornell University > > schul...@cornell.edu > > > > > >
