Looks like a wrong shape for tetrahedral acid ... On May 12, 2015 4:07 PM, "Boaz Shaanan" <bshaa...@bgu.ac.il> wrote:
> Hi, > > > As I've just written to David, maybe it's sulfenic acid (result of > radiation damage?). I've had those in some cases. See attached scheme for > the chemistry and other possibilities for Cys modification by oxidation. > > Boaz > > > > > > > > > > > > *Boaz Shaanan, Ph.D. Dept. of Life > Sciences Ben-Gurion University of the > Negev Beer-Sheva > 84105 > Israel > E-mail: > bshaa...@bgu.ac.il <bshaa...@bgu.ac.il> Phone: 972-8-647-2220 Skype: > boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 * > > > > > > ------------------------------ > *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio > Physics [biophysics.w...@gmail.com] > *Sent:* Tuesday, May 12, 2015 11:57 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] CYS mod > > > Hi All, > > I have same type of modified CYS !!! > No BME. > > On Tue, May 12, 2015 at 2:59 PM, David Schuller <schul...@cornell.edu> > wrote: > >> So far almost everyone is suggesting BME. >> >> I didn't do the purification & crystallization myself, but I am told: >> >> Buffer was HEPES >> cryo was ethylene glycol, glycerol and DMSO. >> Some MgSO4 and KPO4 >> No BME, but maybe some DTT. >> >> Purified from a proteobacterium. >> >> >> >> >> On 05/12/15 15:20, Dyda wrote: >> >>> What is in buffer? Perhaps DTT and cacodylate? >>> >>> Fred >>> >>> >>> [32m******************************************************************************* >>> Fred Dyda, Ph.D. Phone:301-402-4496 >>> Laboratory of Molecular Biology Fax: 301-496-0201 >>> DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov >>> Bldg. 5. Room 303 >>> Bethesda, MD 20892-0560 URGENT message e-mail: >>> 2022476...@mms.att.net >>> Google maps coords: 39.000597, -77.102102 >>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >>> ******************************************************************************* >>> [m >>> >> >> >> -- >> ======================================================================= >> All Things Serve the Beam >> ======================================================================= >> David J. Schuller >> modern man in a post-modern world >> MacCHESS, Cornell University >> schul...@cornell.edu >> > >
